STUDY ON LOW DENSITY LIPOPROTEINS AS AN ALTERNATIVE TO EGG YOLK IN CRYOPRESERVATION OF BUCK SEMEN
| dc.contributor.advisor | G. SUDHA | |
| dc.contributor.author | KAVYASHREE, S. | |
| dc.date.accessioned | 2024-01-09T10:58:17Z | |
| dc.date.available | 2024-01-09T10:58:17Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | The study assessed the seminal attributes of Osmanabadi bucks and effect of replacing egg yolk with different concentrations of LDL on quality of cryopreserved buck semen. Neat semen evaluation of 388 ejaculates from five Osmanabadi bucks over a period of one year revealed mean semen volume (mL), sperm concentration (million/mL), percentage of individual motility, cell membrane integrity, viability, acrosomal integrity and sperm abnormalities as 0.60±0.01, 5119.10±138.44, 81.06±0.37, 85.36±0.24, 86.61±0.23, 85.40±0.24 and 1.09±0.05, respectively. The semen quality reduced significantly during summer season. LDL was extracted from egg yolk and semen extender was prepared with EY (10 %) and different LDL concentrations (6, 8 and 10 % LDL). Semen collected once a week from all bucks were pooled, divided into four groups and cryopreserved with different extenders for a period of 52 weeks (n=52).The seminal parameters at pre-freezing did not vary significantly between the groups, while at post thaw, 8 per cent LDL showed significantly higher acrosomal integrity and lower sperm abnormalities than EY and other LDL groups. The conception rates following AI in 60 non-descript goats using the semen of four different groups (n=15) did not show significant difference between the groups, however, 8 per cent LDL yielded better conception rate (53.33 %) than EY and other LDL extenders. Although, LDL at 8 per cent concentration can be a better alternative to egg yolk in cryopreservation of Osmanabadi buck semen, there is a scope for improvement in LDL extraction process and exploration of alternative ways for its prolonged storage. Key words: LDL, Egg yolk, Osmanabadi bucks, Semen cryopreservation, Seminal attributes, Conception rates. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810206015 | |
| dc.language.iso | English | |
| dc.pages | 184 | |
| dc.publisher | VETERINARY COLLEGE, HEBBAL, BENGALURU KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR | |
| dc.sub | Veterinary Gynaecology and Obstetrics | |
| dc.theme | Veterinary Gynaecology and Obstetrics | |
| dc.these.type | Ph.D | |
| dc.title | STUDY ON LOW DENSITY LIPOPROTEINS AS AN ALTERNATIVE TO EGG YOLK IN CRYOPRESERVATION OF BUCK SEMEN | |
| dc.type | Thesis |