CHARACTERIZATION AND IDENTIFICATION OF CULTIVARS BY USING BIOCHEMICAL AND MOLECULAR MARKERS IN RICE (Oryza sativa L.)
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Date
2017-11-11
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Genetic purity assessment is an important criteria in seed production and seed
certification programme. Therefore, simple and reliable techniques need to be developed
for variety identification and seed genetic purity testing. An attempt was made to
characterize thirty rice cultivars and hybrids along with their parental lines based on their
morphological, biochemical and molecular markers during 2014 to 2017. Seed
morphological characters were used for the broader classification of rice. Both standard
and modified phenol tests with FeSO4 and CuSO4 were found effective in identifying
some of the rice cultivars like MSN-36 (R) from CRMS-32(A) and KRH-4. Besides,
KOH test was useful in identification of red kernel cultivars like CTH-1, CTH-3, JGL-
1798 and Jyothi. The zymogram of total soluble seed protein found to be useful in
distinguishing the rice cultivars, parental lines and hybrids based on the relative intensity
and mobility of bands. Among the tested isozymes, alcohol dehydrogenase (ADH)
banding pattern found useful in differentiating the cultivars like CTH-1, IR-30864 and
IR-64 at Rm value of 0.54 based on the intensity of bands. Out of 84 markers studied, 63
markers found to be polymorphic and 37 markers were unique for the cultivars and
parental lines used in the study. These cultivar specific markers found useful for cultivar
identification and characterization. Seven markers were confirmed hybridity of KRH-2
with its parental polymorphism and in case of KRH-4, 20 markers were confirmed
hybridity of KRH-4 with its parental polymorphism. Unique markers were identified for
the KRH-2 (RM164 and 263) and KRH-4 (RM21, RM 1385, RM 444 and RM 400).
These primers can be effectively used for genetic purity assessment in KRH-2 and KRH-
4. This was the unique study to assess the genetic purity of these line at the seed/seedling
stage and very useful in rice hybrid seed industry.
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