POSTPARTUM RESUMPTION OF OVARIAN ACTIVITY AND ITS ASSOCIATION WITH SUBCLINICAL KETOSIS IN CROSSBRED COWS
Loading...
Date
2019-08-20
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
The aim of the study was Postpartum resumption of ovarian activity
and its association with subclinical ketosis in crossbred cows. The work
was conducted at Instructional Livestock Farm Complex (ILFC), Pookode
and Livestock Research Station (LRS), Thiruvazhamkunnu during the
period of September 2018 to May 2019. The age, parity, milk yield,
peripartum and postpartum complications of the selected animals was
recorded. Crossbred cows on day 30 postpartum, with the history of
peripartum and postpartum complications were screened by rectal
examination at 12 days interval (30, 42 54 66, 78 and 90) for the assessment
of uterine involution and ovarian functional status of the animals. Crossbred
dairy cows at 30 days postpartum were screened for clinical ketosis by
Rothera’s test of urine samples. On day 45 postpartum, the animals without
any clinical signs of ketosis was screened again for subclinical ketosis
(SCK) using cow side BHB testing reagent strips.
Blood samples were collected from animals of both the groups at 12
days interval (30-90 days postpartum). The following biochemical
constituents in serum were analysed in both the groups at 12 days interval
as per standard procedures using diagnostic kits. Total serum cholesterol,
Total lipids, Blood urea nitrogen (BUN), Aspartate aminotransferase
(AST), Serum glucose, BHB. Serum progesterone concentrations analysis
was done in both the groups to assess the resumption of ovarian cyclicity.
Out of 30 animals 40 per cent of animals showed slightly positive, 33.33
per cent of animals showed moderately positive and 8per cent of animals
showed highly positive for Rothera’s test. Control animals observed no
colour change due to absence of ketone bodies. Out of 30 animals
105
200µmol/L, 300µmol/L, 350µmol/L, 400µmol/L and 500µmol/L of BHB
level were present in 13.33, 26.66, 3.33, 53.33 and 6.66 per cent of animals,
respectively. In SCK and non SCK cows mean ± SE of glucose, total
cholesterol, total lipid, AST, BUN and BHB were significantly (P < 0.01)
varied at 12 days interval (30-90 days postpartum). Mean ± SE of glucose,
total cholesterol, total lipid, AST, BUN and BHB in early, late and not
resumed animals were non-significance at 12 days interval (30-90 days
postpartum).
Result indicated that ROA, measured by the appearance of the first
significant progesterone rise ( > 1 ng/ ml in blood ) in early postpartum
period was not associated with SCK .More than 1.2 to 1.4 mmol/L level of
the BHB which indicates presence of SCK. BCS was significantly
associated with SCK and ROA as odds ratios were higher. Biochemical
parameters were associated with the development of SCK, but no
association was observed with postpartum ROA.