Effect of supplementation of zinc and nano zinc on Post thaw variables and oxidative status of bovine semen
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Date
2023-06
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Abstract
The present study was undertaken to investigate the effect of supplementation of zinc and
nano zinc on post thaw variables and oxidative status of bovine semen The concentration of ZnCl2 and
nano ZnO was optimized for use in semen extender. 22 semen ejaculates from three indigenous cattle
bulls were supplemented with the optimized concentration of ZnCl2 (40mg/ml) and ZnONP (10 and 20
µg/ml) and were cryopreserved as per the standard freezing protocol. Semen was evaluated for
morphological, motility and antioxidant parameters. Microbial load was also evaluated. It was observed
that the nanoparticles supplementation significantly increased pre and post freezing motility parameters
of semen in groups 1 and 2 in comparison to control group and group 3. Post thaw seminal parameters
viz., intact acrosome, plasma membrane integrity and percent live sperms were found to be
significantly better in semen samples supplemented with nanoparticles with 20 µg/ml as compared
group 1, 3 and control. Lipid peroxidation levels reduced, significantly in all supplemented groups as
compared to control and ROS levels significantly reduced in samples supplemented with 20 µg/ml
NPs. Level of glutathione peroxidase and reductase was significantly reduced in group 2 supplemented
with 20 µg/ml NPs as compared to control. Microbial load of frozen thawed semen sample was
evaluated to check the growth of common contaminants of semen samples by spread plate method. The
NP supplemented semen samples had very less bacterial colonies as compared to control and group 3.
Thus it can be concluded that nano ZnO as a supplement in the bull semen extender can be used to
enhance the quality of semen as well as to replace the use of antibiotics in semen extender.