In Vitro and In Vivo Propagation of Difficult-to-Root Bougainvillea Cultivars
Loading...
Date
2014
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Division of Floriculture and Landscaping Indian Agricultural Research Institute New Delhi
Abstract
Bougainvillea sp. is commonly propagated by hardwood cuttings but this method
is tedious and time consuming. Moreover there are certain cultivars where the rooting is
very low. For easy, quick, and mass multiplication of such cultivars, tissue culture
technique can be put to use. Present investigation was carried out with two objectives.
First, to standardize a protocol for in vitro multiplication of two difficult-to-root
bougainvillea cultivars, viz., Mahatma Gandhi and Refulgens and second, was to test the
suitability of air-layering to enhance rooting in difficult-to-root cultivars. In the in vitro
experiment, nodal segments with axillary buds were used as explant. For culture
establishment, MurashigeSkoog medium supplemeneted with BAP (5 mg/l) was found
to be the best with highest culture establishment (81.13%) and the fastest bud sprout
(8.18 days). Murashige and Skoog medium supplemented with BAP (4.0 mg/l) and
kinetin (0.5 mg/l) gave the highest shoot proliferation (av 4.11 micro-shoots/explant) in
both the cultivars. The best treatment for micro-shoot elongation was the one where MS
medium was supplemented with 0.5 mg/l GA3 giving highest elongation (3.89 cm).
Highest in vitro rooting (64.99%) of micro-shoots was noted in the treatment having
half-strength MS medium supplemented with 1.0 mg/l IBA. Hardening was most
effective in glass jar with polypropylene cap. The hardened plantlets were successfully
transferred to the glasshouse after a short period of in vitro acclimatization.
The in vivo experiment results revealed that almost all cultivars showed
significant difference in rooting percentage, growth, and flowering. In difficult-to-root
cultivars such as Sweet Heart, Red September, Thimma, , Mahatma Gandhi, Refulgens
and Dr. H.B. Singh good rooting (59.33, 59.02, 53.66, 50.66, 47.98% and 43.33,
respectively) was obtained through the use of air-layering during june- july Month with
1000 ppm IBA in lanolin paste. Maximum rooting and survival of plants (100.00%) was
recorded in Chitra and Dr. R.R. Pal cultivars, which are easy-to-root. The biochemical
investigation of stem tissue revealed that these cultivars showed that the easy to root
cultivars have higher carbohydrate content (40.58 and 23.96 %) and lower nitrogen
content (2.24 and 2.54 %) than the difficult-to-root ones.
40
tM +&fuekZ.k esa dfBu cksx sufofy;k
Description
t-9090
Keywords
In Vitro and In Vivo Propagation of Difficult-to-Root Bougainvillea Cultivars