Diversity Analysis and Integrated Disease Management of Xanthomonas oryzae pv. oryzae causing Bacterial Leaf Blight of Rice (Oryza sativa L.)
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Date
2021
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INDIRA GANDHI KRISHI VISHWAVIDYALAYA RAIPUR (Chhattisgarh)
Abstract
The present experiment entitled ‘‘Diversity Analysis and Integrated Disease Management of Xanthomonas oryzae pv. oryzae causing Bacterial Leaf Blight of Rice (Oryza sativa L.)’’ was conducted in the Department of Plant Pathology, College of Agriculture, IGKV, Raipur (C.G.) during 2020-21.“Bacterial leaf blight (BLB) disease is caused by Xanthomonas oryzae pv. oryzae and is one of the most devastating”diseases. There is no reliable source of resistance against this disease. Moreover, Xoo have a vast diversity in their population. BLB samples were collected and isolated from different geographical areas of Chhattisgarh, i.e., Raipur, Mahasamund, Dhamtari and Ambikapur. The pathogen producedglighthyellow,ushiny, viscous,ksmooth, and circularmcolonies on potato sucrose agar (PSA) medium. Tested the pathogenicity on TN1 variety of rice were found the typical symptom of bacterial blight was observed as yellow lesions on both leaf margins, leaving a green area in the centre. Bacterial ooze was “seen on the margins or veins of the freshly infected leaf under moist conditions”in the morning hours, and it is a source of secondary inoculums. In the present investigation variation in the virulence spectrum was observed and was speculated due to the presence or absence of avr gene in the pathogen. The presentoinvestigation wasrthereforedcarriedsoutaby speculatinggthexavr genesmin thevpathogenzbased onbthecphenotypichresponsesuof NILs”carrying gene and gene combinations. Host specific / incompatible interactions of the isolates on the near isogenic lines (NIL’s) indicated highly resistance / hypersensitive responseskaremclearly thehresult of the molecularjcross talklbetween avirulenceygene producty(s)land correspondingfRngenekproduct (s) therefore basedpon information of avr gene mapping one can get an idea about the gene or gene combination effective in an area. Such information is essentially required for conventional breeding, marker assisted breeding or gene (s) deployment strategies. Thirty NILs and pyramids (IRBB lines) along with susceptible check, TN1 were evaluated for monitoring the diversity and field virulence response of four Xanthomonas oryzae pv. oryzae (Xoo) isolates, only four NILs i.e. IRBB-56, IRBB-57, IRBB-60 and IRBB-66 effective at all 4 locations. Different ages of Xoo culture were observed, the culture of Xoo at 120 hours and 72 hours were found most effective in terms of disease severity and produced highly susceptible reactions on TN1. Screened thirty nine rice entries out of thirty nine rice entries, twenty eight entries, expressed highly resistant reactions against all Xoo isolates i.e. Raipur, Mahasamund, Dhamatari and Ambikapur. Evaluation of nano silver particles (Borogold) forftheomanagementpoftbacterial leaf blight disease in rice. Among all the seven treatments, the maximum reduction in disease severity (15%) and percent disease reduction over control treatment (69.90%) were recorded in T2.
Description
Diversity Analysis and Integrated Disease Management of Xanthomonas oryzae pv. oryzae causing Bacterial Leaf Blight of Rice (Oryza sativa L.)