GROSS, HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON THE OVIDUCT OF ADULT RAJASRI FOWL
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Date
2024-02
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SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502. (A.P.) INDIA
Abstract
The present study was conducted on thirty six(36) apparently healthy laying
Rajasri fowls. The study was carried out in laying birds of same age group ranging from
26 to 28 weeks. The average lengths and weights of oviduct and its five major divisions
like infundibulum, magnum, isthmus, uterus and vagina were individually measured.
The histomorphological and histochemical characteristics of the oviduct was
studied by using light microscope. The wall of oviduct composed of tunica mucosa,
lamina propria sub mucosa, tunica muscularis and tunica serosa from inside to outside.
The length of mucosal fold and height of epithelium and lamina propria submucosa and
thickness of tunica muscularis of different segments were measured by using ocular and
stage micrometer under light microscope.
The tunica mucosa of funnel part of infundibulum was projected into mucosal
folds which were lower in height when compared to posterior part of infundibulum in
laying Rajasri fowl. The luminal epithelium was lined by simple columnar epithelium and
consists of non ciliated cells, ciliated cells and goblet cells. The lamina propria sub
mucosa was extended into the core of mucosal folds, but the tubular glands were absent.
The tunica muscularis was well represented with inner circular and outer longitudinal
smooth muscle layers. The tunica serosa which was outermost layer was comprised of
loose connective tissue and it was lined by simple columnar epithelium. The mucosal
folds in magnum were leaf like in shape and are longer in length when compared to
infundibulum. It was lined by pseudostratified ciliated columnar epithelium along with
non ciliated cells. The lamina propria submucosa was packed with long branched tubular
glands. These mucosal folds of isthmus were lower in height when compared to magnum.
The thickness of tunica muscularis was almost similar to that of magnum and it contained
nerve fibres and blood vessels in between the layers of muscle fibres. The lamina propria
submucosa of uterus was consisted of highly vascularized loose connective tissue with
branched tubular glands. The tubular glands were densely packed and were separated
from each other by collagen, reticular and nerve fibres. The tunica muscularis of uterus
was thicker than the other parts of oviduct in laying Rajasri fowl. The tunica mucosa of
vagina was raised into numerous longitudinal mucosal folds and these are narrow when
compared to other parts of oviduct in laying Rajasri fowl. The sperm host glands were
observed in uterovaginal junction and the cytoplasm of this sperm hostage glands was
vacuolated with lipid inclusions. The inner circular smooth muscle layer of vagina was
strongly developed and forms the vaginal sphincter at the opening of cloaca.
The apices of lining epithelium of mucosal folds in infundibulum, magnum,
isthmus, uterus and vagina of oviduct showed the PAS strong +VE reaction indicating the
presence of mucopolysaccharides in laying Rajasri fowl. They also showed the positive
for alcian blue and indicated the presence of acid mucopolysaccharides. The glycogen
content was observed at the apical regions of the non secretory cells in the lining
epithelium of mucosal folds of oviduct in laying Rajasri fowl.