Some studies on the microflora in preputial washings and semen of buffalo bulls

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Date
1991
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
Delay in puberty causes great economic losses to dairy farmers. The present study was planned to see the effect of gonadotropins and gonadotropin releasing hormones in delayed pubertal buffalo-heifers and thus to bring them into estrus. Seventy-five percent of the buffalo-heifers responded to GnRH administration whereas 87.5 per cent response was seen with PMSG and HOG administration. The plasma estradiol-17 maximum during estrus Phase whereas plasma praesterone level was maximum during the diestrus phase of the estrous cycle. The plasma FSH and LH levels were found to be maximum during the estrus phase of the cycle. There was increase in the thyroid gland activity with the onset of estrus cyclicitv resulting in higher circulating levels of plasma triiodo­thyronine and thyroxine. The plasma, cortisol level did not show much variations. The hemoglobin packed cell volume, blood glucose and total protein, inspite of slight variations, remained unaffected with the administration of these hormones. The plasma total cholesterol was high during pre-treatment period and during the estrus phase of the induced estrous cycle. Various plasma enzyme activities were estimated and there was not much variations in the plasma enzyme concentrations. However, slight variations did occur with the change in reproductive status of the buffalo-heifers. The plasma macro-and micro-elements did not show much variations. These findings lead us to the conclusion that various hormonal parameters showed significant variations with the onset of estrus cyclicity. These variations were under the normal range of concentration during different phases of estrous cycle in this species. However, other biochemical constituents did not show much variations with the administration of exogenous hormones and remained within the normal range. The use of these hormones were found to be helpful for bringing the delayed pubertal buffalo-heifers Intorus cyclicity and thus making them capable of carrying out various process of reproduction
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