GENOMIC EXPRSSION OF CERTAIN PRO INFLAMMATORY CYTOKINES DURING TRANSITION PERIOD IN ONGOLE CATTLE (Bos indicus)
Loading...
Files
Date
2024-02
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502. (A.P.) INDIA
Abstract
The present study was designed to evaluate the effect of transition stress in ongole
cows during transition period. Twelve animals (n=12) were randomly selected from
livestock research station (LRS), Lam farm, Guntur, Andhra Pradesh of which 6 are
multiparous pregnant animals (transition group) and 6 are clinically healthy heifers
(control group). From gravid females, samples were collected during transition period
week-3, week-2, week-1, on the day of parturition, week+1, week+2, week+3.
Physiological parameters like RT, RR and PR were recorded daily at 9:00 AM throughout
the study period. Three aliquots of blood sample (whole blood with anticoagulants in two
vacutainers and one vacutainer of blood with clot activators for serum isolation) were
collected. One aliquot of whole blood was directly used for analysis of haematological
parameters (TEC, TLC, Hb, PCV, Platelets, DLC, Erythrocyte indices) and blood glucose,
while another aliquot was used for separation of PBMC and subsequent RNA isolation
for gene expression studies of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), HSP90.
Blood with clot activators was processed to separate serum, for estimation of biochemical
parameters (total protein, albumin, cholesterol, ALT, AST, Creatinine, BUN, Calcium,
Phosphorous).
The mean±SE values of RT, RR and PR vary significantly (p<0.05) between
control and transition group. The mean±SE values of RT, RR and PR didn’t vary
significantly (p>0.05) in between weeks of transition group. The mean±SE values of
haematological parameters vary significantly (p<0.05) between control and certain weeks
in transition group. The mean±SE values of haematological parameters like platelets,
MCHC, granulocytes and monocytes vary significantly (p<0.05) within transition group
and also vary significantly between the control and transition group and significant
(p<0.05) difference was observed within the weeks of transition group and between
control and transition group.
The mean±SE values of albumin and creatinine did not vary significantly (p>0.05)
between control and transition group and no significant (p>0.05) difference was observed.
The blood glucose levels vary significantly (p<0.05) between control and transition group
when compared to control group the levels of glucose decreased in transition weeks and
the blood glucose also vary significantly (p<0.05) within the weeks of transition group,
the blood glucose levels decreased significantly from prepartum to on the day of
parturition and then increased significantly from the day of parturition to postpartum and
significant (p<0.05) difference was observed between control and transition group and
within the weeks of transition group. The mean±SE values of ALT were significantly
(p<0.05) higher in postpartum when compared to control. The mean±SE values of AST
vary significantly (p<0.05) within the weeks of transition group and significant (p<0.05)
difference was noticed within the weeks in transition group. The mean±SE values of AST
were significantly (p<0.05) higher in prepartum week-2, week-1, 0day and postpartum
weeks in comparison to control. The mean±SE values of total protein and globulin were
significantly (p<0.05) higher in week-3, week-2 and week+1 of transition group in
comparison to control. The mean±SE values of BUN, calcium, phosphorous were
significantly (p<0.05) lower on week-2 in transition group when compared to control.
The mean±SE values of BUN vary significantly (p<0.05) within the weeks in transition
group and significant (p<0.05) difference was within the weeks of transition group. The
mean±SE values of cholesterol were significantly (p<0.05) lower on week-2, week-1,
0day, week+1 in transition group in comparison to control.
The relative mRNA expression of IL-1β was significantly upregulated in
transition group on prepartum 0 day and postpartum week+1, week+2, week+3 compared
to control and the expression of IL-1β was Significantly (p<0.05) downregulated in
transition group compared to control group on week-3. The relative mRNA expression of
IL-6 was not significantly (p>0.05) different between control group and animals in week
3, week-2, week-1, 0day, week+1, week+2 and week+3. The relative mRNA expression
of TNF-α was significantly(p<0.05) upregulated in transition group on prepartum week
2, 0 day and postpartum week+3 compared to control. The relative mRNA expression of
TNF-α was significantly (p<0.05) downregulated in transition group on week+1
compared to control. The relative mRNA expression of HSP90 was significantly (p<0.05)
downregulated in transition group compared to control group in prepartum week-3, week
1, and postpartum week+1, week+2, week+3. No significant (p>0.05) difference was
observed between the control group and week-2, 0 day of transition group.