Web blight of mungbean (Vigna radiata L.) and its management
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Date
2022
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Punjab Agricultural University, Ludhiana
Abstract
Web blight of mungbean, caused by Rhizoctonia solani, has a significant impact on grain yield and quality. The pathogen is soil-borne and has a broad host range. Field surveys were conducted in different districts of Punjab during the year 2019-20. The survey concluded that Ludhiana district had the lowest prevalence and severity of web blight, while Ballowal Saunkhri (SBS Nagar) had the most. Pathogen variability was investigated at morphological and molecular level. On the basis of morphological characterization viz. colour, mycelial abundance, shape and size of sclerotia, the pathogen was identified as Rhizoctonia solani. Of all the five isolates, BS (Ballowal Saunkhri) and NS (Nawanshahr) were found to be the fast-growing isolates with a 0.88 mm/hr growth rate followed by RO(Ropar) and L1(Ludhiana 1) and L2 (Ludhiana) 0.68 mm/hr. Among all the isolates, L1-L2, BS-L1, RO-L1, NS-L1, and NS-BS were vegetatively compatible, whereas BS-L2, NS-L2, BS-RO, RO-NS, RO-L2 were vegetatively incompatible. The isolates were characterized on the basis of ITS region sequence homology and confirmed as R. solani. The sequences have been registered with NCBI as accession numbers ON17668, ON176686, ON084814, ON084815 and ON084816. Further, Among the three inoculation techniques viz. soil inoculation, foliar spray and field screening at hotspot location, the foliar spray technique was found to be the most precise inoculation technique. Among the four biocontrol agents viz. recommended consortium (LSMR 1+RB3), a new consortium (Bacillus subtilis +RB1), Trichoderma viride, and Pseudomonas flourescens, the new consortium (Bacillus subtilis +RB1) was found most effective against web blight under both in vitro and in vivo conditions. Further, a set of 58 elite mungbean genotypes was screened for web blight resistance by field screening technique at hotspot location. Out of 58 genotypes, seven namely, SML 2061, SML 2056, SML 2064, SML 2055, SML 2073, SML 2065 and SML 2070 showed resistant reaction to web blight.
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Raghveer Singh (2022). Web blight of mungbean (Vigna radiata L.) and its management (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.