Studies on Hericium erinaceus (Bull.:Fr)Pers. and its cultivation on locally available substrates in sub-tropical zones of Himachal Pradesh
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Date
2024-11-12
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College of Horticulture and Forestry Dr YSP UHF, Neri, Hamirpur(H.P.)
Abstract
The investigations titled " Studies on Hericium erinaceus (Bull.:Fr)Pers. and its cultivation on locally available substrates in sub-tropical zones of Himachal Pradesh." were carried out at the Mushroom Research Laboratory, Department of Plant Pathology, Dr Y. S. Parmar University of Horticulture and Forestry, College of Horticulture and Forestry, Neri, Hamirpur, Himachal Pradesh during 2022-2024. The study focused on investigating physiological aspects for vegetative growth of different strains of H. erinaceus, antimicrobial activity of different strains of H. erinaceus against various phytopathogenic fungi along with evaluating various locally available substrates for production of different strains of H. erinaceus. The results of present investigation revealed that out of eight different nutrient media, potato dextrose agar proved best medium for mycelial growth (38.71 mm) however, modified potato dextrose broth yielded maximum mycelial biomass (411.22 mg) of test fungus. Out of seven different pH levels under evaluation, maximum mycelial growth (52.02 mm) was recorded at pH level 8.5 in all the strains of test fungus. Among five different temperature regimes under study, 25℃ proved to be the most favourable temperature for vegetative growth (40.31 mm) of all the strains of H. erinaceus. Maximum mycelial biomass production of all the H. erinaceus strains under study was recorded in sorbitol (103.55 mg), calcium nitrate (144.85 mg) and magnesium sulphate (122.22 mg) as carbon, nitrogen and trace element, respectively. Out of three strains under study, H. erinaceus strain DMRX-780 exhibited fastest vegetative growth in all the nutrient media, different pH levels and different temperature regimes. All the strains of H. erinaceus exhibited antifungal activity against Phomopsis psidii, Pestalotiopsis mangiferae, Alternaria brassicicola, Fusarium species and Colletotrichum truncatum while, no inhibition was recorded against Aspergillus and Rhizopus species. Among six different grain substrates evaluated, maximum linear growth of all the strains of test fungus was recorded in wheat grain substrate therefore, wheat was selected as best spawn grain substrate for spawn production of all the H. erinaceus strains under study. Out of six different agroforestry production substrates, shortest period (19.33-32.00 days) for spawn run was recorded in tooni sawdust substrate in all the H. erinaceus strains. However, the fruiting was observed only in strain DMRX-779 and tooni sawdust substrate was found to be the best for cultivation of this strain as it took shortest time for pin head initiation (22.67 days) and first flush (29.00 days) along with producing maximum number (5.00) and yi,ld (150.33 g/kg dry substrate) of fruit bodies with 15.03 per cent biological efficiency. No fruiting was observed in strains DMRX-778 and DMRX-780 up to 55 days of incubation.