Molecular analysis of recombinant inbred lines for Karnal bunt (Neovossia indica) resistance in wheat
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Date
2012
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CCSHAU
Abstract
Present study was carried out t o screen 107 recombinant inbred l in es (RILs) of cross
H567. 71 (r esistant) x WH542 (susceptible) for Karnal bunt resistance and to identify and validate
SSR markers associated with Karnal bunt resistance in wheat. There was significant variation
among all the recombinant in bred lines for percentage and coefficient of infection to Karnal bunt.
Most of the RILs (85) and (72) showed up to 5% infection during the y ear 2010 and 2011 and thus
were resistant. The range of percentage of infection (PI) in the susceptible RILs was 5.19 to 22.93 and
5.38 to 36.46 in the year 2010 and 2011 respectively , whereas, coefficient of infection on RILs
ranged f r om 0 t o 8. 28 and 0 t o 14. 45 in the yea r 2010 and 2011 respectively. Significant variation s
were also observed for different morphological traits such as, plant height, spike length, days to
flowering, till er s/plant , 100 grain weight and grain yield/ plant. A total of 70 SSR markers were
used t o screen parental genotypes out of these 42 were polymorphic. These 42 SSR markers were
used to screen 20 selected RILs and a fingerprinting database was generated. Total of 88 alleles
were detected, number of alleles per locus ranged from 2-3 with an average of 2.09 alleles per locus.
Genetic similarity analysis showed that parental genotypes were quite distinct from each other . The
cluster analysis led to distribution of parents and their RILs in to two groups at the similarity
coefficient value of 0.39. Major cluster I, included resistant parent H567.71 with resistant lines and
major cluster II had susceptible parent WH542 and susceptible lines. Bulk segregant analysis showed
that three prime rs (Xgwm637, Xgwm337, Xgwm538) which were already reported to be associated
with Karnal bunt resistance, also showed association with Karnal bunt resistance in population under
study . A new marker (Xgwm6) located at 1.6 cM from Xgwm538 (o n chromosome 4B) was identif ied
to be associated with karnal bunt resistance. These four markers were fur the r used to screen o n whole
population and results were confirmed. Hence, these markers may be used for marker assisted selection
for Karnal bunt resistance in early segregating generations .
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Keywords
RILs, Karnal bunt, SSR markers