Effect of exogenous Lipopolysaccharides (LPS) of different strains of Rhizobium on nodulation in chickpea (Cicer arietinum L.)
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Date
2009-09-17
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University of Agricultural Sciences GKVK, Bangalore
Abstract
The present investigation was carried out to study the effect of exogenous
Lipopolysaccharides (LPS) of different strains of Rhizobium on nodulation in chickpea
(Cicer arietinum L.). The lectin was isolated from chickpea seed and root to see the effect
on Lipopolysaccharides (LPS) which were isolated from four chickpea specific
Rhizobium strains namely IC-59, IC-1148, IC-2091 and IC-149 and one non specific
Rhizobium strain P-132. The strain specific lipopolysaccharides of specific strains of
Rhizobium inhibited lectin induced agglutination of Rhizobium specific to chickpea. And
agglutination was observed higher in case of seed lectin than that of root lectin, as there
was no agglutination with non specific strain so no inhibition of lectin was observed. This
was also supported by the binding study data that chickpea root lectin binds more
strongly to homologous Rhizobium than seed lectin. So that higher concentration is
needed for replacement of lectin from Rhizobium. Number of lectin binding sites per
Rhizobium is three to four times more with respect to chickpea specific strains as
compared to non specific strain. Nodulation experiment showed all the chickpea specific
Rhizobium strains formed nodules in in vitro experiment whereas non specific strain does
not form any nodules. This follows the dictum all or none phenomenon. In other words,
nonspecific Rhizobium does not inhibit agglutination and nodulation upon addition of
exogenous LPS
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