Strain Typing of Mycobacterium avium subsp. paratuberculosis from Tamil Nadu, India based on Polymorphisms in MAP 1506 Locus and IS 1311 PCR-REA
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Date
2015-05
Journal Title
Journal ISSN
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Publisher
Advances in Animal and Veterinary Sciences
Abstract
Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease, a progressive chronic granulomatous enteritis affecting the domestic ruminants. Dung samples from cattle, sheep and goat were collected from the suspected clinical cases in livestock farms of different regions of Tamil Nadu state, India. All the samples were subjected to IS 900 PCR for the detection of MAP and 45% were found to be positive. All the positive samples were subjected to IS 1311 PCR-REA that confirmed MAP ‘Bison type’ is most prevalent cause of JD among the domestic ruminants in Tamil Nadu. MAP isolation has been attempted from the tissue samples collected from the animals that were slaughtered being positive for JD. A total of 4 isolates were obtained and were characterized by IS1311 PCR-REA, MAP1506 PCR and sequence analysis which identified the single nucleotide polymorphisms that allow strain typing. One isolate from sheep intestine was identified as “Intermediate type” strain by MAP 1506 PCR-sequence analysis. To the best of our understanding, this is the first report of ‘Intermediate type’ strain of MAP from sheep from India.
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Keywords
Mycobacterium avium subsp. paratuberculosis, Strain typing, Tamil Nadu, IS 1311 PCR-REA, MAP1506 PCR and sequence analysis