Styrene Maleic Anhydride (Sma) as a male Contraceptive: a Novel non Hormonal Approach in Dog and Rat
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Date
2020-02
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Abstract
The present work involves the development of a novel method of contraception in male animals using
a synthetic co-polymer of Styrene Maleic Anhydride (SMA). For the polymer preparation, a simple single
pot RAFT polymerization technique was carried out. The 2-dodecyl-thiocarbonothioyl-thio-propanoic acid
(PADTC) was synthesized in the first step which was then used as a catalyst to initiate the polymerization
process between styrene and maleic anhydride. The prepared SMA was analyzed using NMR, FTIR, MALDITOF,
Zeta potential analyzer for its physical properties which proved to be similar to the commercially available
polymer. The in vitro viability and integrity of canine spermatozoa were tested using different concentration
of polymer. The motility, acrosomal integrity, mitochondrial membrane potential and DNA integrity before
and after incubation were tested and the results indicated that 2 mg dissolved in100 μl DMSOproduced
necrozoospermia in 60 minutes. The micro structural alterations of the sperm were confirmed using Atomic
force microscopy (AFM). The invivo studies were performed using bilateral injection of polymer hydrogel
into the vas deferens of adult male Wistar rats (n=6). Those injected rats were cohabitated with female rats
(1:2) post 15 days of surgery and proved to produce no litters. The results indicated that the SMA formed
a chemical mesh inside vas deferens which was evident through Scanning Electron Microscope (SEM) and
causes electrolytic interaction in the sperms passing through it and hence rendering it infertile for a conception.
In conclusion, SMA would be a quantum leap in modern contraception when it comes to mass sterilization
of stray dogs.
Description
TNV_20thMVC_PP_Feb-2020_OP47
Keywords
Veterinary Science