GENETIC STRUCTURE OF Semecarpus kathalekanensis: A CRITICALLY ENDANGERED TREE SPECIES OF CENTRAL WESTERN GHATS
| dc.contributor.advisor | VASUDEVA, R | |
| dc.contributor.author | SURAJ, P G | |
| dc.date.accessioned | 2019-10-24T07:10:37Z | |
| dc.date.available | 2019-10-24T07:10:37Z | |
| dc.date.issued | 2001 | |
| dc.description.abstract | Genetic structure of Semecarpus kathalekanensis , a crtically endangered, dioecious medicinal tree species of the Myristica swamps of the central Westem Ghats was studied adopting isozyme and PCR based Randomly Amplified Polymorphic DNA (RAPD) techniques. Leaf samples from 34 adult individuals of two neighboring populations were subjected to starch-gel electrophoresis after standardizing the protocol for six isozyones. Only four isozyme loci out o f 9 identified were polymorphic in both the populations. The overallmean allele number per loci was 1.44. The Shannon's information index based on all loci was 0.204 ± 027; it was 0.55 ±0.11 based on polymorphic loci. The observed mean heterozygosity (Ho) of the populations was extremely low at 0.095 compared to the expected heterozygosity (He) based on Hardy-Weinberg equilibrium (0.134). This strongly suggests that the populations are suffering from heterozygote deficiency, which could be attributed to severe genetic bottleneckmg and genetic drift. Rare-relic nature and severe anthropogenic pressures faced by the species might be responsible for genetic drift, while, lack of mating partners due to incomplete blooming synchrony among male and female trees could result in inbreeding. Interestingly, bigger matured individuals with GBH (>100 cm) had higher Ho (11.11%) than those w'hich had less than 100 cm GBH (6.67%) indicating that younger generations might posses reduced genetic variation, which calls for an urgent recovery plan. The genomic DNA were initially subjected to PCR based RAPD using 65 primers. A total of 76 loci were identified based on 10 random primer data. The polymorphism for RAPD ranged from 71.05 to 61.84 per cent and a diversity index of 0.627. The level of genetic differentiation among two populations was very low as suggested by mean Fst value (<1%). This study, perhaps the first assessment of genetic diversity of a critically endangered tree species in India, strongly suggests that understanding genetic structure of rare/endangered species is highly essential in formulating conservation plans. | en_US |
| dc.identifier.citation | No . of references 128 | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810132784 | |
| dc.keywords | GENETIC STRUCTURE OF Semecarpus kathalekanensis: A CRITICALLY ENDANGERED TREE SPECIES OF CENTRAL WESTERN GHATS | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 143p. | en_US |
| dc.publisher | University of Agricultural Sciences, Dharwad | en_US |
| dc.research.problem | GENETIC STRUCTURE OF Semecarpus kathalekanensis: A CRITICALLY ENDANGERED TREE SPECIES OF CENTRAL WESTERN GHATS | en_US |
| dc.sub | Forest Biology and Tree Improvement | en_US |
| dc.subject | null | en_US |
| dc.theme | GENETIC STRUCTURE OF Semecarpus kathalekanensis: A CRITICALLY ENDANGERED TREE SPECIES OF CENTRAL WESTERN GHATS | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | GENETIC STRUCTURE OF Semecarpus kathalekanensis: A CRITICALLY ENDANGERED TREE SPECIES OF CENTRAL WESTERN GHATS | en_US |
| dc.type | Thesis | en_US |