Study of variability induced through mutagenesis in chickpea (Cicer arietinum L.)
| dc.contributor.advisor | Arora, Anju | |
| dc.contributor.author | Dinkar, Vishal | |
| dc.date.accessioned | 2018-09-06T10:19:11Z | |
| dc.date.available | 2018-09-06T10:19:11Z | |
| dc.date.issued | 2015-07 | |
| dc.description.abstract | Chickpea (Cicer arietinum L.) is a self-pollinated diploid (2n=16) annual grain legume that belongs to the family Fabaceae. Due to self-pollinated nature of crop and small flower size, conventional methods of plant breeding have a limited scope in improvement of chickpea. Mutation breeding is an effective tool for a plant breeder to generate variability especially in crops like chickpea with narrow genetic base. The present investigation was carried out in three desi chickpea varieties - Pant Gram 114, Pant Gram 186 and Pant Gram 3 released from Pantnagar. The seeds were mutagenized with gamma ray (300 Gy & 400 Gy doses), ethyl-methane sulphonate (EMS 0.5%) and their combination treatments (300 Gy gamma ray + 0.5% EMS & 400 Gy gamma ray + 0.5% EMS). M1 generation showed gradual decrease in germination with increase in the mutagen strength in all the three varieties which indicated that combination treatments caused more biological damage than individual mutagen treatment. Morphological mutants such as chlorophyll mutants, high anthocyanin pigmented type, narrow leaves with entire margin types, variegated leaf type, tall type and fasciated stem type were observed. In M2 generation, chlorophyll mutations were most frequent in Pant Gram 186 followed by Pant Gram 3 and Pant Gram 114. The interaction effects were found significant with significant effects of varieties for characters viz., plant height, days to first flower, height of first pod bearing node from base, days to maturity, number of secondary branches /plant, leaf length and yield/plant. This indicated that varietal differences were significantly affected by different mutagenic treatments. In M2 generation, individual plant progenies showed segregation for different characters. Among all the three varieties, PG3 M1 plant progenies were found more frequently segregating i.e., 11%, followed by PG186 (9%) and PG114 (7%). In all the three varieties, frequency of viable mutants increased with increase in dose of gamma ray, whereas EMS alone gave comparatively higher frequency than combined treatments. Viable mutants were more frequent in PG3 variety i.e., 1.04% followed by PG186 (0.69%) and PG114 (0.54%).In PG114, significant change of mean from control was observed only for plant height, days to maturity, internode length, cumulative length of petiole and rachis, leaf length, leaf width, pod length. In PG186, for most of the characters change in mean was significant except for plant height, no. of secondary branches/plant, cumulative length of petiole and rachis, average no. of seeds/pod and yield/plant. In PG3, mean for most of the characters decreased except days to flowering and no. of secondary branches/plant. In all three varieties, variance for yield and most of the yield contributing characters significantly increased in all kind of mutagenic treatments except for plant height and height of first pod bearing node (in PG114), average no. of seeds/pod (in PG186) and cumulative length of petiole and rachis; average no. of seeds/pod and yield/plant (in PG3). In general, combined treatments 300 Gy + 0.5% EMS and 400 Gy + 0.5% EMS were observed to be most effective in inducing variability (high variance and CV) for most of the characters under study. In a nutshell, it can be concluded that combined treatments 300 Gy + 0.5% EMS and 400 Gy + 0.5% EMS were observed to be most effective in inducing variability (high variance and CV) for most of the characters under study. Different viable mutants identified in individual plant progeny rows in M2 generation like fasciated stem type, long internode type, leaf mutants, growth habit mutants like primary branching mutant, secondary branching mutant, compact type, tall types, bushy types and erect types, late maturity type, flowering mutant, and double mutants (i.e., light green plant colour type, tall + erect types, tall + early flowering type and early flowering + leaf mutant type) can be used as genetic stocks for useful traits and can be utilized in future crop improvement programs. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810072166 | |
| dc.keywords | variability, mutagenesis, chickpeas, Cicer arietinum | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 102 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Chickpeas | en_US |
| dc.sub | Genetics and Plant Breeding | en_US |
| dc.subject | null | en_US |
| dc.theme | Induced Mutation | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Study of variability induced through mutagenesis in chickpea (Cicer arietinum L.) | en_US |
| dc.type | Thesis | en_US |