Characterization of potential Bradyrhizobium/ Ensifer strains for improving biological nitrogen fixation and yield in soybean [Glycine max (L.) Merrill]
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Date
2013
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PAU Ludhiana
Abstract
he present investigation was carried out to study the characterization of potential Bradyrhizobium/Ensifer strains for improving biological nitrogen fixation and yield in soybean [Glycine max (L.) Merrill]. Nine authentic strains of rhizobia were collected from different AICRP centres of soybean in India. Out of nine strains, 5 were identified as slow grower Bradyrhizobium strains (IND1, LSBR3, PANT1, SB271 & DS1) and 4 strains (IND2, LSER7, LSER8 & PANT2) as fast grower on the basis of their morphological and biochemical characteristics. Further, These strains were investigated for their functionality traits viz. indole acetic acid (IAA), phosphate (P) solubilization hydrogenase activity and intrinsic antibiotic spectra. Significantly high IAA was recorded with LSER8 (25.75µg ml-1) in the presence of L-tryptophan (0.01%). Significantly high P solubilization of TCP (100 mg) in Pikovaskaya’s medium was observed with IND2 (5.80 mg 100 ml-1) at 12th day. In qualitative screening of hydrogenase activity 2 Bradyrhizobium strains (LSBR3 and DS1) and 3 Ensifer strains (LSER7, LSER8 and PANT2) showed red coloration on YEMA media amended with 0.01% TTC dye. Roots from soybean seeds bacterized with Bradyrhizobium strain LSBR3 produced significantly high amount of flavonoid like compounds. Potential of different rhizobial strains were further evaluated for symbiotic efficiency, growth improvement and grain yield in soybean (SL744) under field conditions. Significantly high growth parameters, symbiotic traits and grain yield was observed with two Bradyrhizobium strains (LSBR3 and PANT1) and Ensifer strain (LSER8). These rhizobial strains can be explored as future biofertilizer to promote growth and yield in soybean.
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