Upscaling of lignocellulolytic enzyme production from biodigested slurry for increasing biogas production from paddy straw

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dc.contributor.advisorGupta, Urmila
dc.contributor.authorNavdeep Kaur
dc.date.accessioned2021-07-30T04:54:02Z
dc.date.available2021-07-30T04:54:02Z
dc.date.issued2021
dc.description.abstractThe objective of the present study was to upscale the process of microbial production of lignocellulolytic enzymes using biodigested slurry as a substrate followed by biogas production from paddy straw using crude lignocellulolytic enzymes. Quantitative screening of the fungal cultures namely Coriolus versicolor MTCC 138, Pleurotus ostreatus MTCC142, Phaenarochaete chrysosporium MTCC 787 and Rhizopus sp indicate that exoglucanase (0.095U/ml), manganese peroxidase (0.305U/ml) and lignin peroxidase (0.437U/ml) activities were maximum in Coriolus versicolor followed by Pleurotus ostreatus. and Phaenarochaete chrysosporium while β-glucosidase activity was maximum in Phaenarochaete chrysosporium. Enzyme production from Coriolus versicolor and Delftia PP4_S3 were statistically optimized by using box-behnken design of response surface methodology (RSM) by Statgraphics software. Coriolus versicolor MTCC 138 produced maximum enzymes at 50% slurry concentration; 3% inoculum; 7 pH; 30˚C temperature and 8 days of incubation. The optimized conditions for maximum enzyme production by Delftia PP4_S3 were: 50% slurry concentration; 1% inoculum; 37˚C temperature and 5 days of incubation. The enzyme production studies were conducted in the field fermentor of 500l capacity using Coriolus versicolor, Pleurotus ostreatus and Delftia PP4_S3. Maximum exoglucanase, β-glucosidase, manganese peroxidase and lignin peroxidase activities were found on 7th day of incubation in Pleurotus ostreatus and Coriolus versicolor while in Delftia PP4_S3 on 4th day of incubation. The crude enzyme produced using BDS was supplemented with paddy straw and was used for biogas production in MS steel biogas plant of 400Kg capacity. The biogas produced by paddy straw supplemented with Delftia crude enzyme was 101m3 as compared to control of 65m3 while biogas produced by paddy straw supplemented with Pleurotus ostreatus crude enzyme was 30m3 where control produced only 27m3. Thus, biodigested slurry is a stable substrate for lignocellulolytic enzymes which can efficiently increase biogas production.en_US
dc.identifier.citationNavdeep Kaur (2021). Upscaling of lignocellulolytic enzyme production from biodigested slurry for increasing biogas production from paddy straw (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810171120
dc.keywordsLignocellulolytic enzyme production, Biodigested slurry, Quantitative screening, Optimization, Response Surface Methodology, Upscaling, Biogas production, Paddy strawen_US
dc.language.isoEnglishen_US
dc.pages116en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemUpscaling of lignocellulolytic enzyme production from biodigested slurry for increasing biogas production from paddy strawen_US
dc.subMicrobiologyen_US
dc.themeUpscaling of lignocellulolytic enzyme production from biodigested slurry for increasing biogas production from paddy strawen_US
dc.these.typeM.Scen_US
dc.titleUpscaling of lignocellulolytic enzyme production from biodigested slurry for increasing biogas production from paddy strawen_US
dc.typeThesisen_US
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