Upscaling of lignocellulolytic enzyme production from biodigested slurry for increasing biogas production from paddy straw
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
The objective of the present study was to upscale the process of microbial production of
lignocellulolytic enzymes using biodigested slurry as a substrate followed by biogas
production from paddy straw using crude lignocellulolytic enzymes. Quantitative screening of
the fungal cultures namely Coriolus versicolor MTCC 138, Pleurotus ostreatus MTCC142,
Phaenarochaete chrysosporium MTCC 787 and Rhizopus sp indicate that exoglucanase
(0.095U/ml), manganese peroxidase (0.305U/ml) and lignin peroxidase (0.437U/ml) activities
were maximum in Coriolus versicolor followed by Pleurotus ostreatus. and Phaenarochaete
chrysosporium while β-glucosidase activity was maximum in Phaenarochaete
chrysosporium. Enzyme production from Coriolus versicolor and Delftia PP4_S3 were
statistically optimized by using box-behnken design of response surface methodology (RSM)
by Statgraphics software. Coriolus versicolor MTCC 138 produced maximum enzymes at
50% slurry concentration; 3% inoculum; 7 pH; 30˚C temperature and 8 days of incubation.
The optimized conditions for maximum enzyme production by Delftia PP4_S3 were: 50%
slurry concentration; 1% inoculum; 37˚C temperature and 5 days of incubation. The enzyme
production studies were conducted in the field fermentor of 500l capacity using Coriolus
versicolor, Pleurotus ostreatus and Delftia PP4_S3. Maximum exoglucanase, β-glucosidase,
manganese peroxidase and lignin peroxidase activities were found on 7th day of incubation in
Pleurotus ostreatus and Coriolus versicolor while in Delftia PP4_S3 on 4th day of incubation.
The crude enzyme produced using BDS was supplemented with paddy straw and was used for
biogas production in MS steel biogas plant of 400Kg capacity. The biogas produced by paddy
straw supplemented with Delftia crude enzyme was 101m3 as compared to control of 65m3
while biogas produced by paddy straw supplemented with Pleurotus ostreatus crude enzyme
was 30m3 where control produced only 27m3. Thus, biodigested slurry is a stable substrate for
lignocellulolytic enzymes which can efficiently increase biogas production.
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Citation
Navdeep Kaur (2021). Upscaling of lignocellulolytic enzyme production from biodigested slurry for increasing biogas production from paddy straw (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.