Studies on standardisation of macropropagation technique for Dalbergia sissoo Roxb
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Date
2024
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Punjab Agricultural University
Abstract
In the present study, the effect of cutting size , position, clonal variability and IBA levels on the macro propagation and growth of plantlets in Dalbergia sissoo were evaluated . In the first experiment, shoots of clone 5 of Dalbergia sissoo were collected from the clonal hedge orchard maintained at Research Farm of the Department of Forestry and Natural Resources, Punjab Agricultural University, Ludhiana . The cuttings of different sizes 4 cm, 7 cm and 10 cm ) were treated with 4 hormonal treatments (0 ppm, 1000 ppm, 2000 ppm and 3000 ppm IBA) and we re planted in the mist chamber conditions. The significant differences were found among cutting sizes and IBA levels for rooting and sprouting percentage, number of roots, root length, shoot length, shoot biomass and root biomass. The 7 cm cutting size sho wed the maximum rooting percentage (70.50 %) which was statistically at par with 4 cm cutting size (70 %) and 2000 ppm had the highest rooting percentage (74.45 %) which was statistically similar to 1000 ppm IBA level (73.33 %) In second experiment, cutti ngs from different positions (top, middle and basal) were treated with 3 hormonal treatments (0 ppm, 1000 ppm and 2000 ppm IBA) and were planted in the mist chamber conditions. The significant differences were found among the cutting positions and IBA leve ls for rooting percentage, number of roots, root length, shoot length, shoot biomass and root biomass. C uttings taken from middle portion had maximum rooting (66.67 %). With regard to IBA levels, maximum rooting was observed at 2000 ppm treatment (66.67 %) which was statistically similar to 1000 ppm IBA level (64.17 %). In third experiment, s hoots from 15 different clones from the clonal hedge orchard maintained at Research Farm of the Department of Forestry and Natural Resources, Punjab Agricultural Univers ity, Ludhiana were collected in order to make cuttings
for evaluating the variability in the rooting behaviour of Dalbergia sissoo clones. Significant differences were observed among 15 clones of Dalbergia sissoo for sprouting, rooting and growth traits. Clone 5 was found to be the best with respect to rooting percentage (61.25 %). The genetic parameters were worked out for various rooting and growth traits.
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Harmandeep Kaur (2024). Studies on standardisation of macropropagation technique for Dalbergia sissoo Roxb (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.