Light and ultrastructural studies on the blood cells of Uttara fowl
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Date
2014-06
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was conducted on the blood cells of ten Uttara fowl. The nucleated erythrocytes were elliptical or oval in shape with mean size 11.20±0.25 µm in length and 6.99±0.14 µm in width. The heterophils were round in shape with mean diameter 9.07±0.55 µm and had spindle or banana shaped light pinkish granules in the cytoplasm. The eosinophils were 9.21±0.60 µm in mean diameter with rounded and strongly eosinophilic cytoplasmic granules. The basophils were round in shape with mean diameter 9.38±0.55 µm and had metachromatically stained cytoplasmic granules. The small, medium and large lymphocytes were observed with mean diameter 5.80±0.12 µm, 7.00±0.22 µm and 10.92±0.20 µm respectively. The monocytes were the largest leucocytes observed in the blood of Uttara fowl, with mean diameter 11.83±0.44 µm. Thrombocytes were pleomorphic but usually round in appearance with mean diameter 4.89±0.20 µm. The heterophils showed weak positive reaction for SBB, alkaline phosphatase and mild reaction for cytochrome oxidase and peroxidase. The eosinophils revealed strong positive reaction for glycogen, general lipids, cytochrome oxidase, peroxidase and weak positive reaction for acid and alkaline phosphatase. The basophils showed strong positive reaction with toluidine blue stain. Four types of leucocytes were observed under SEM and the thrombocytes were roughly round or oval in shape having varying sized cytoplasmic processes. The erythrocytes under transmission electron microscope were elliptical or oval in shape. Reticulocytes were also observed having mitochondria and polysomes within the cytoplasm. The cytoplasmic granules of heterophils and eosinophils were pleomorphic in appearance. The cytoplasmic granules of basophils were generally of two types - round electron dense and honey comb like. Lymphocytes were almost round in shape with numerous cytoplasmic processes and pseudopodia. Monocytes had kidney or dumbbell shaped nucleus with finger like cytoplasmic processes on cell surface. The thrombocytes were nucleated containing cell organelles close to nuclear membrane and peripheral ectoplasm with ribosomes and glycogen granules.
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