PHENOTYPIC AND MOLECULAR GENETIC CHARACTERIZATION OF DAHLEM RED LAYERS
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Date
2006-03
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
Thc investigation was made on full sib, half sib and non-inbred groups of Dahlem Red
birds maintained at the Project Directorate on Poultry. Hyderabad. to characterize then
phenotypically and by RAPD markers. The phenotypic characterization war undertaken on a total of 328 Dahlem Red birds belonging to full sib mated (45). half sib mated (64) and non-inbred groups (219). The overall
icast squares means of body weight at 4. 8. 20, 40. weeks of age. ASM. EP 40. EW 32 and
EW 40 were 146.88. 374.28. 1150.70. 1678.57 g. 181.02 days, 71.06, 52.81g and 56.25 g.
rcspectivoly. The mean performance of non-inbred group was in general. superior to inbred
groups in dl the traits studied. The estimates of heritability obtained by half sib mehod for the full sib. half sib and non-inbred groups were 0.02. 0.61 and 0.49. respectively for BW 4 and 0.19, 0.20 and 0.53.
rrspectrvely for BW 40. For egg weights. the highest heritabilities were obtained in half sib
group and the lowest in non-inbred group. Majority of the genetic comlations of BW 20 md BW 40 wih egg weight were positive. The genetic comlii~ionosf ASM with EP 40 and egg weights were negative. The EP
40 was also found to be correlated negatively with EW 32 and EW 40.
Majonfy of tht phcnotyp~cc omlations between the body weight and egg we~ghtw ere
positive, while che comlations of ASM with EP 40 were negative. The phenotyp~c
conrlarions of ASM with EW 32 and EW 40 mgcd from -0.18 to 0.17. where&\ the
comlations between EP 40 and egg weights were negative in half sib and non-inbred grvups
* - - - -- Fn an --A ,,, ...-:nt,, 0 13 The phenotypic comlations estimated by full sib method among the body weights
were in general positive and ranged from -0.11 t 0.34 in full sib group. The BW 4 and BW 8
were comlated negatively with ASM. while BW 20 and BW 40 were also correlatd
negatively with EP 40. The phenotypic correlations of BW 4. BW 8, and BW 40 with egg
weights were found to be positive. The ASM was comlated negatively with EP 40 (-0.58).
whereas, the correlations of EP 40 with EW 32 and EW 40 were positive.
The phenotypic comlations among the body weights in half sib group ranged from
-0.06 to 0.46; The phenotypic correlations between body weights and ASM varied from -0.19
to 0.30, whereas the comlations of body weights with EP 40 ranged from -0.10 to 0.34. The
phenotypic correlations between body weights and egg weights at various ages were all
positive. For molecular genetic characterization, a total of 48 Dahlem Red birds. ( 16 birds each
of the group) with equal representation of sexes, were utilized. A total of 100 Random
Amplified Polymorphic DNA (RAPD) primers were tested, of which, 21 primers generated
appreciable polymorphism. A total of 341 RAPD bands were produced, out of which 204
wen found to be polymorphic. The overall mean number of bands produced by the primer
varied from 12 to 23. The size of amplified bands varied from 100 bp to 3409 bp.
The analysis of the fingerprint patterns of individual DNA samples revealed that the
full sib group was unique by the pre.xnce of one 1866 bp fingerprint when amplified by the
primers PDP157F and 496 bp and 380 bp fingerprints only when amplified by the primer
PDP176F. The half sib group wa\ unique by the presence of 1788 bp fingerprint when
amplified by the primer PDPSOF. The non-inbred group could be distinguished by the
prcsence of a unique 42 1 hp band bawd upon PCR amplification with primer PDP54F and
PDP73F. The overall genetic sirn~lwitiesh a.sed on band sharing within the full sib, half sib and
non-lnhred group were 87.90. 87.85 and 83.94 per cent. respectively, whereas the genetic
similarities between full sib and half sib, half sib and non-inbred and full sib and non-inbred
groups were 90.18. 88.51 and 88.07 percent, respectively. The overall genetic similarities based on band frequency within full sib, half sib and non-inbred groups were 85.25. 85.09 and 8 1.14 per cent, respectively, whereas those between full sib and half sib, half sib and non-inbred and full sib and non-inbred groups were 79.53,
74.34 and 72.78 per cent, respectively. The genetic distance between full sib and non-inbred
groups was found to be the maximum (0.3 176). while the minimum genetic distance (0.2290)
was observed between full sib and half sib groups. The RAPD markers in the present study
were able to detect the polymorph~sm between the three groups of Dahlem Red i.e., full sib
mated, half sib mated and non-inbred and could establish the genetic relationships
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