Pharmacognostic studies and evaluation of anti-inflammatory, analgesic and antioxidant potential of ‘Manjakantha’ (Dracaena terniflora Roxb.)
| dc.contributor.advisor | Latha, P G | |
| dc.contributor.author | Meera, Mohan | |
| dc.contributor.author | KAU | |
| dc.date.accessioned | 2017-07-12T07:30:20Z | |
| dc.date.available | 2017-07-12T07:30:20Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | : The study entitled “Pharmacognostic studies and evaluation of antiinflammatory, analgesic and antioxidant potential of ‘manjakantha’(Dracaena terniflora Roxb.)” was conducted at the Ethnomedicine and Ethnopharmacology Division of Jawaharlal Nehru Tropical Botanical Garden (JNTBGRI), Palode, Thiruvananthapuram, during the year 2015 - 2016. The main objective of the study was to evaluate the anti-inflammatory, analgesic and antioxidant potential of the ethanolic extract of the root of the ethnomedicinal plant Dracaena terniflora Roxb. and to carry out its pharmacognostic studies In the detailed study of pharmacognostical parameters, the microscopic evaluation showed the presence of anomalous secondary thickening in Dracaena terniflora roots (Monocot), in powder analysis microscopically scattered tracheids and vessels are seen ash value was low as 12.84%, moisture is obtained as 62.03%. Differential extractive value of Dracaena terniflora roots were carried out in which the highest extractive yield was obtained in acetone, which was 7.5% and then Preliminary phytochemical screening revealed the presence of various phytoconstituents like flavonoids, steroids, saponins, alkaloids, carbohydrates and phenols with different extracts. Total flavonoid of 500μl sample is 0.1026μg/ml and phenolic content in 500μl sample is 1.4115μg/ml. In in vitro antioxidant method the methanolic extracts of root showed higher free radical scavenging activity compared to standards with EC50 of 304.98μgml-1 (DPPH), 484.68 μgml-1 (NO Scavenging Activity) and 528.43 μgml-1 (Hydroxyl Free Radical Scavenging Activity). Toxicity of the ethanolic root extract of Dracaena terniflora were tested by acute toxicity study in mice with four doses 25, 100, 400,1600mgKg-1 body weight. And the mice were caged and observed for fourteen days and no toxic effect were seen in the tested animals. Detailed in vivopharmacological studies for anti-inflammatory and analgesics were conducted. Anti-inflammatory activity was determined by Carrageenan (Acute inflammation) and formalin (sub chronic inflammation) induced paw oedema on hind limb in rats with three different doses of ethanolic root extract of D.terniflora with 50, 150 and 450 mgKg-1. At the dose of DT 450 mgKg-1 give maximum inhibition of 63% in carrageenan induced paw oedema and 84% and 96%inhibition in first and seventh day of formalin induced paw oedema study respectively. Analgesic activity was conferred in Eddy’s Hot Plate method and Acetic acid induced writhing method in mice with three different doses of 50, 150 and 450 mgKg-1. At the dose of DT450mgKg-1 gives maximum inhibition 67.4% of inhibition in Eddy’s hot plate method and 62.31% in the acetic acid induce writhing study. The anti-inflammatory and analgesic property of D. terniflora may be due to the presence of the secondary metabolites present in it, such as flavonoid & phenolic compounds, polysaccharides, steroids, triterpenoids, alkaloids, tannins etc. The results of current study will help to develop a monograph of the drug by keeping it as a reference. These results substantiate the traditional claim of the plant for its anti-inflammatory action. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810025092 | |
| dc.keywords | Plant biotechnology | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | College of Agriculture, Vellayani | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | anti-inflammatory, analgesic and antioxidant potential | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Pharmacognostic studies and evaluation of anti-inflammatory, analgesic and antioxidant potential of ‘Manjakantha’ (Dracaena terniflora Roxb.) | en_US |
| dc.type | Thesis | en_US |