Investigating plausible role of AHL gene-family in drought stress tolerance in rice (Oryza sativa L.)
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Date
2023
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RPCAU, Pusa
Abstract
The transcription factor AHL (AT-hook motif nuclear localized) are one of the largest and most conserved gene families in plants and they play critical roles in abiotic stress response. However very limited information available about the AHL family of genes in rice. In this context, a genome wide survey, was done to identify the AHL genes in rice (Oryza sativa L.) and total 20 members AHL family of genes were identified. The distribution of these 20 genes were on the 9 chromosome of rice. Mostly AHL genes transcription factors were located in nucleus. The phylogenetic analysis conducted for these 20 genes of AHL TFs and it divided into two major groups and four subgroups. To study the role of AHL group of genes in drought and salt stress at seedling stage of rice. The two genotypes IR64 & NL44 were chosen for gene expression study. The genotypes were grown in pot. The fourteen days old seedlings were exposed to 20 % PEG (2 hours and 4 hours) and 200 Mm NaCL (2 hours and 4 hours) for drought and salt treatments respectively. The mRNA of each treatment of 14 days old seedlings extracted in the replication and cDNA synthesized. Quantitative real time PCR based expression analysis of AHL genes in IR64 genotype of rice under drought and salinity stresses. The expression pattern of AHL family of genes was compared by one-way ANOVA in IR64 genotype. The result clearly showed that the OsAHL group of genes in drought and salt stress tolerance. Further, differential expression of AHL family gene was studied in IR64 and NL44 under drought and salt stress conditions. Total 8 genes (OsAHL1, OsAHL2, OsAHL3, OsAHL10, OsAHL14, OsAHL17, OsAHL18 and OsAHL19) were showed significantly more expression in NL44 as compared to IR64 after 2 hours of drought treatment. Similarly, all total 7 genes (OsAHL7, OsAHL10, OsAHL13, OsAHL14, OsAHL17, OsAHL18 and OsAHL20) showed significantly more expression in NL44 as compared to IR64 after 4hours drought treatment. Total 3 genes (OsAHL10, OsAHL13 and OsAHL20) showed significantly up-regulated after 4hours salinity treatment in cultivar NL44 as compare to IR64. Overall, these findings indicate that AHL genes might be involved in mediating drought and salt signaling transduction pathways when plants are exposed to drought and salt stress. The NL44 could be used as a potential donor for drought and salt stress tolerance.