Standardisation of clonal propagation and variation studies in different species of bamboo in coastal Odisha
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Date
2018-07-01
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OUAT,Bhubaneswar
Abstract
The present experiment entitled “Standardization of clonal propagation and variation studies
in different species of Bamboo in coastal Odisha” was conducted with the objectives to study the
response of different species of bamboo (Dendrocalamus strictus, Bambusa vulgaris, Bambusa
nutans, Bambusa tulda and Bambusa bambos) to various concentrations of IBA for shoot and root
initiation of culm cutting method and to study the phenotypic variations of sprouts and roots
regenerated from culm cuttings among the 5 species of bamboo due to variations in their genetic
behaviour in order to get an idea for mass multiplication and biomass production to meet the
multifarious demands of bamboo based sector. .
The experiment was carried out in Split-Plot Design with 3 replications with 5 species of
bamboo as main plot factor and different concentrations of IBA (0 ppm, 100 ppm, 200 ppm, 300
ppm) as sub-plot factors. The findings revealed that maximum sprout height (58.85 cm) was observed
in B. vulgaris. Maximum height growth was recorded in B. vulgaris for 100 ppm (72.3 mm)
.Maximum Collar Diameter growth (5.08 mm) among 5 species was observed in B. vulgaris and
Highest collar diameter was observed in B. vulgaris for 100 ppm (5.8 mm). Maximum no. of leaves
(11.05) was observed in B. vulgaris. B. vulgaris for 0 ppm (13.1) was observed to be most suitable
hormone combination. Longest leaf length was observed in B. tulda (15.38 cm). B. tulda with 300
ppm was recorded longest leaf (18.2 cm). Maximum leaf breadth (6.2 cm) was observed in B. nutans.
Widest leaf breadth was observed in B. nutans (6.3 cm) for 200 ppm. Maximum number of shoots
(6.86) was observed in B. nutans. B. bambos for 300 ppm (8.9 cm) was recorded highest number of
shoots. Maximum number of nodes (7.87) was observed in B. vulgaris. Highest number of nodes was
recorded in B. tulda for 100 ppm (9.2 cm). Maximum branching (7.55) was observed in B. tulda.
Highest number of branches was recorded in B. tulda for 200 ppm (10.3). Maximum number of roots
(55.3) was observed in B. nutans. Highest number of roots was recorded in B. nutans for 0 ppm
(55.3). Maximum root length (36.7 cm) was recorded in B. nutans. Longest root length was recorded
in B. nutans for 300 ppm (36.7 cm). Maximum fresh shoot biomass (170.06 gm) was observed in B.
nutans. 0 ppm for B. nutans (275.1 gm) was recorded maximum fresh shoot biomass. Maximum dry
shoot biomass was observed in B. nutans (86.67 gm). Maximum fresh root biomass was observed in
B. nutans (54.4) for 300 ppm. Maximum Quality Index was recorded in B. nutans for 0 ppm (7.8).
Maximum Volume Index was observed in B. vulgaris for 200 ppm (2442.8). Highest correlation was
observed between Fresh Root Biomass and Dry root Biomass (0.98) and lowest correlation was
observed in between no. of roots and collar diameter (0.42) followed by Dry shoot Biomass and Root
Length (0.47). Phenotypic correlation of characters was ranged between 0.413 (no. of nodes and no.
of shoots) to 0.996 (fresh shoot biomass and dry shoot biomass).
Description
TH 5463
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