Micropropagation studies in ber (Ziziphus mauritiana Lamk.)
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Date
2007
Authors
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Journal ISSN
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Publisher
CCSHAU
Abstract
The experiment was conducted during
2005-2006 on “Micropropagation studies in ber (Ziziphus mauritiana
Lamk.)” in the laboratory of Centre for Research and Application in
Plant Tissue Culture CRAPTC CCS HAU, Hisar Haryana. The efforts
were made to optimize conditions for mass-multiplication of ber
cultivars Gola and Umran. The explants comprised of shoot tip and
nodal segment with different combination of growth regulators and
additives (BAP, NAA, IBA, Kinetin, KNO3, NH4NO3) supplementing the
MS basal medium. Sterilization treatment of 0.1% HgCl2 supplemented
with ascorbic acid and citric acid for 3 minutes duration was observed
best for cvs. Gola and Umran. The minimum number of days taken for
shoot initiation was observed in MS+BAP 0.5 mg/l in both cultivars.
The maximum shoot regeneration, shoot length and average number of
shoots per plant was observed on MS+ KNO3 3800 mg/l + NH4NO3
2475 mg/l + BAP 2.5 mg/l + IAA 0.5 mg/l media in cvs. Gola and
Umran. The shoots were transferred for rooting on different rooting
media. Rooting was not observed in both cultivars on any of the rooting
media tried.
Description
Keywords
Ziziphus mauritiana Lamk., Micropropagation