MERISTEM CULTURE OF APPLE CULTIVAR ‘OREGON SPUR-II’
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Date
2013
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Abstract
ABSTRACT
In the present study, an attempt was made to eliminate ACLSV, ApMV,
ASGV and ASPV from apple cultivar ‘Oregon Spur-II’. Thermotherapy was carried
out at 37-40°C for 4 weeks followed by culturing of meristems of different sizes.
During establishment of explants, highest survival percentage (62.35%) and
proliferation (30.68%) was recorded during summer season, due to less browning and
contamination. However, size of meristems and position of buds from where
meristems were excised also influenced their survival. The meristems of size 0.6 – 0.7
mm were found to be the most appropriate for maximum establishment i.e., 36.36 %.
Meristems excised from buds positioned on distil portions of actively growing shoots
showed better results. MS medium supplemented with BA (1.0 mg/l), IBA (0.05 mg/l)
and GA3 (0.1 mg/l) resulted in 56.62% establishment of explants, while maximum
number of meristems proliferated with low BA (0.5 mg/l), IBA(0.08mg/l) and same
GA3 concentration. Two to four fold multiplication was observed. Virus indexing of
shoots raised from different sizes of meristems was carried out and found that 0.3-
0.6mm size was able to eliminate ACLSV, ApMV, ASGV and ASPV. However,
some of 0.5-0.6 mm sized shoots were found infected with ACLSV. Larger meristems
could not completely eliminate the viruses under study.