Interaction of Bt cotton (Gossypium hirsutum) with native rhizospheric and inoculated microflora
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Date
2008
Authors
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Publisher
CCSHAU
Abstract
In the present study, effect of Bt cotton was studied on native and
inoculated microorganisms in the rhizospheric soil under pot house as
well as field conditions. Marginal increase in native microbial population
of non-Bt cotton compared to Bt cotton after 45 DAS of sowing was
observed in two different varieties RCH134 and MRC6301 tested.
However, after 90 days of sowing there was decrease in microbial
population and it was almost similar both in the Bt and non-Bt cotton
rhizosphere. The inoculation of Azotobacter (A-2), Azospirillum (J-11-12)
and PSB (BPL-2) increased the rate of seed germination both in Bt as
well as non-Bt cotton in the variety RCH 134. However, the effect was
more pronounced in non-Bt cotton as compared to Bt cotton.
Supplementation of root exudates of Bt and non-Bt cotton at a
-iiconcentration
of 0, 2 or 4% in Jensen, malate and Pikovaskaya media
resulted in increase in growth rate of A-2, J-11-12 and BPL-2 as
compared to control. Genetically marked strains of Azotobacter (A-2),
Azospirillum (J-11-12) and phosphate solubilizing bacterium (BPL-2)
established better in the rhizosphere of non-Bt cotton, var. RCH134
under pot house conditions and their population was ten times higher
after 30 days of sowing. Increase in plant height, plant biomass, N and
P-uptake due to inoculation was observed at recommended dose of
fertilizers both in Bt as well as in non-Bt cotton.
Description
Keywords
Cotton, Planting, Bacteria, Exudates, Transgenics, Vegetative propagation, Biological development, Proteins, Application methods, Sowing