Enzyme and Antioxidant Profile of Follicular Fluid in Sheep
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Date
2016-03
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TANUVAS
Abstract
The follicular fluid consists of locally produced substances that act as regulatory
factors in follicular development and steroidogenesis. Among the various constituents,
alkaline phosphatase, lactate dehydrogenase and antioxidants in the follicular fluid provide
information about the metabolic activities and degenerative changes that take place in the
cells during follicular growth. Hence, this study was conducted to evaluate the enzyme
and antioxidant concentrations of follicular fluid in relation to follicular size. About two
hundred and fifty pairs of ovaries of Madras Red sheep in apparently good health were
randomly collected after slaughter from Corporation Abattoir: Chennai. Follicular fluid was
aspirated from small (less than 2 mm), medium (2-4 mm) and large (greater than 4 mm)
ovarian follicles and centrifuged in a cooling centrifuge at 40C at 3000 rpm for 15 minutes.
The supernatant fluid from the three categories of follicles was collected and analyzed
for alkaline phosphatase, lactate dehydrogenase, antioxidant enzymes such as superoxide
dismutase, catalase and glutathione peroxide as per the standard protocol. The data were
analyzed statistically by ANO VA. It was observed that the alkaline phosphatase and lactate
dehydrogenase activity significantly decreased (P < 0.05) as the follicle size increased. The
activity of superoxide dismutase and catalase significantly decreased (P < 0.05) with an
increase in follicle size whereas, glutathione peroxidase activity significantly increased (P <
0. 05) with increased follicle size.
Description
TNV_IJVASR_M-A2016_V45_I02_Pg543-548
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