Genetic analysis of seed yield and quality traits and molecular characterization for white rust resistance in Indian Mustard [Brassica juncea L. (Czern. & Coss.)]
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Date
2023-08-03
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CSK HPKV, Palampur
Abstract
The p esent st entit e “Geneti ana sis o see ie an q a it t aits an mo e a ha a te ization
for white rust resistance in Indian Mustard [Brassica juncea L. (Czern. & Coss.)]’’ was a ie o t to
congregate information on genetic architecture, combining ability, heterosis and inheritance of white rust
resistance. The materials for the investigation comprised of 12 lines, three testers (RCC-4, Donskaja-IV and
their F1) and resulting 36 TTC hybrids. These progenies and their parents were grown in randomized
complete block design with three replications during rabi 2021-22 at the Experimental Farm of the
Department of Genetics and Plant Breeding, CSK HPKV, Palampur (H.P.).
Analysis of variance indicated the significance of mean squares due to genotypes for all the traits
which revealed the presence of sufficient genetic variability in the existing material. Modified triple test
cross analysis exhibited the presence of epistasis for all the traits namely, days to flower initiation, days to 75
per cent maturity, plant height, reproductive phase, number of primary branches per plant, number of
secondary branches per plant, siliquae per plant, length of main shoot, siliquae length, seeds per siliqua,
1000-seed weight, seed yield per plant, biological yield per plant and harvest index except for days to 50 per
ent owe ing. F the , pa titioning o epistasis e ea e the impo tan e o ‘j ’ t pe o epistasis o a most
all the t aits ex ept a s to 50 pe ent owe ing whe eas mean sq a es e to ‘i’ t pe inte a tion we e non significant for most of the traits except number of secondary branches per plant, siliquae per plant, siliqua
length, seeds per siliqua, seed yield per plant and biological yield per plant which shows the importance of
both ‘i’ t pe an ‘j ’ t pe o epistasis. Re ati e magnit e o a iti e omponent D) was p e ominant o e
dominance component (H) for most of the traits except reproductive phase, number of secondary branches
per plant, siliquae per plant and biological yield per plant indicating the relative importance of fixable type
of gene action in their inheritance.
Analysis of variance for line× tester revealed the presence of substantial genetic variability.
Analysis of variance for combining ability indicated significant differences among hybrids for all the traits
studied. The genotype DRMR 2017 was found to be good general combiner for nine traits followed by
RSPR-03 for eight traits and Pusa Mehak for seven traits. Five crosses viz., Pusa Tarak × Donskaja-IV,
Kranti × Donskaja-IV, RSPR-03 × Donskaja-IV, DRMR 2017 × RCC-4 and Urvashi × Donskaja-IV
exhibited higher per se performance, significant SCA effects and heterobeltiosis for seed yield per plant.
Two crosses namely, Urvashi × Donskaja-IV and Pusa Tarak × F1 showe ow g osino ate ontent ˂30
µmole per gram of defatted seed meal) and only one genotype viz., Pusa Karishma showed low erucic acid
˂ 2 pe ent).
To study the inheritance of white rust resistance, two crosses (RCC-4 × Donskaja-IV and RCC-4 ×
Heera) were evaluated. In cross RCC-4 × Donskaja-IV, dominant epistasis (12:3:1) was observed while in
cross RCC-4 × Heera, complementary epistasis (9:7) was observed. Molecular characterization revealed that
intron polymorphic (IP) markers viz., At5g41560 and At2g36360 were highly linked to white rust resistance
loci AcB1-A4.1 and AcB1- A5.1 and could be helpful in marker assisted selection for white rust resistance
in Indian mustard.