IDENTIFICATION OF MOLECULAR MARKER FOR INTROGRESSION OF MUNGBEAN YELLOW MOSAIC VIRUS (MYMV) RESISTANT IN MUNGBEAN (Vigna radiata L.)
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Date
2014-01
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Navsari Agricultural University, Navsari
Abstract
Twenty six germplasm lines were screened at the hot
spot of Pulse Research Stat ion (PRS), Navsari for the finding
resistant source of MYMV. Four germplasm lines viz.,
NAUMR1, NAUMR2, NAUMR3 and MEHA showed highly
resistant react ion against YMD. NAUMR1, NAUMR2 and
NAUMR3 were found late maturing, tall and produced few
pods only. MEHA is MYMV resistant mungbean cult ivar
developed and released from IIPR, Kanpur in 2004, however,
is of small size. Out of 200 RAPD markers, only fourteen
markers showed variable degree of polymorphism. Maximum
90% polymorphism was observed in OPG-5, OPJ-18 and OPM-
20 whereas minimum polymorphism (28.57 per cent) was in
OPH-9. Overall polymorphism was found to be 70.17 per cent.
Fourteen markers on five genotypes generated 126 bands out
of which 90 were polymorphic band. OPG-5, OPJ-18 and
OPM-20 were proved to be the best markers for the study of
polymorphism as it produced 28, 35, 28 amplicons
respect ively. In OPG-5 a band of size ~850bp was absent in
suscept ible cult ivar GM4 while present in all the resistant
lines. In the marker OPJ-18, total 6 unique band of
ABSTRACT
Abstract….
approximately 250bp, 300bp, 450bp, 500bp, 850bp and 1250bp
size were present in all resistant lines while absent in
suscept ible one. Simi larly in the marker OPM-20 3 exclusive
band 350bp, 400bp and 600bp size appeared in all resistant
lines whereas absent in suscept ible GM4. The dendrogram
obtained with range of similarity coefficient 0.41 to 0.79
clearly indicated two dist inct main clusters A and B. Cluster A
included suscept ible GM4 and cluster B showed the all
resistant lines viz., NAUMR1, NAUMR2, NAUMR3 and
MEHA. Cluster B bifurcated in to two subclusters, B1 and B2
with simi larity coefficient of approx. 0.72. Subcluster B1
comprises MEHA whereas subcluster B2 contained remaining
three resistant lines. Diversity in all the resistant and
suscept ible were observed by the ISSR markers also. Out of
the seventeen RGA marker combinat ions, four markers
amplified DNA from the resistant, however, could not ampli fy
any DNA fragment from the suscept ible. On sequencing al l
these fragment were found 98 % similarity with each other.
Similarly in the case of other RGA fragments showed
simi larity with Vigna radiata viral resistance candidate. In F1
all the plants showed resistant phenotype and also the
presence of specific band of ~450bp in molecular analysis
with the RGA markers which was absent in the suscept ible
cult ivar GM-4. The same followed phenotypic as well as
genotypic segregat ion rat io of 3 (Resistant ):1(Suscept ible) in
F2. Interest ingly in BC1F1, 10 plants were observed and the
1:1 rat io of phenotype and genotype was observed. Data
suggested that the resistance is governed by single dominant
gene. Looking at the phenotypic, genotypic and agronomic
characterist ics, MEHA was found superior among all the
Abstract….
resistant sources. Therefore MEHA is further proposed for the
int rogression of resistant gene through breeding programme.
Use of the cult ivated variety in breeding as donor is always
preferred over uncharacterized germplasm as to reduce linkage
drag. Among four markers, RGA pair-2 (VRMYMVRGA-2) is
recommended to check the inheritance of gene in succeeding
generat ions.
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Keywords
diseases, genes, planting, crossing over, rapd, selection, polymorphism, land resources, germplasm, dna