Biochemical and histochemical analysis of black pepper (Piper nigrum L.) inoculated with mycorrhiza from different crop management systems

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Date
2008-08-08
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University of Agricultural Sciences GKVK, Bangalore
Abstract
The investigation was carried out to study the biochemical, histochemical analysis of Piper nigrum L. and Sorghum bicolor (L.) Moench inoculated with mycorrhiza from different crop management systems. Black pepper and sorghum were inoculated with mycorrhiza from the organic soil, inorganic soil, natural soil, control and Glomus fasciculatum. There were three experiments in which the first experiment was on growth response of black pepper to the inoculation of mycorrhiza from different crop management systems. In second experiment black pepper and sorghum were inoculated with mycorrhiza from different crop management systems and in third experiment sorghum was inoculated with mycorrhiza from different crop management systems. Histochemical analysis of these crop plants were carried out to study the difference in the accumulation of the polysaccharides, proteins and nucleic acids in leaf tissues of control and mycorrhiza inoculated plants. Plants inoculated with Glomus fasciculatum showed highest rooting percentage, percent root colonization, phosphorus, potassium and nitrogen content. Plants inoculated with Glomus fasciculatum recorded increased biomass than control. A significant difference in the accumulation of polysaccharides, proteins and nucleic acid content was observed in leaf sample of the plant inoculated with Glomus fasciculatum.
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