EFFECT OF EXTENDER AND DIFFERENT SPERM NUMBERS PER STRAW ON QUALITY OF FROZEN SEMEN IN BEETAL AND SIROHI BUCKS
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Date
2016-01
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Assam Agricultural University, Khanapara, Guwahati
Abstract
A total of 80 pooled ejaculates collected from two Beetal and one Sirohi bucks maintained at Goat Research Station, Burnihat were used to study the effect of tris extender containing 20% egg-yolk, 1% soy-lecithin and 1.5% soy-lecithin, and that of 37.5, 50 and 75 x106 sperm / straw on the quality of frozen semen, and also the effect of number of spermatozoa in frozen semen straws on fertility. The freezing of semen was done in French mini straw by rapid horizontal vapour freezing technique using liquid nitrogen.
The overall mean post-thaw per cent sperm motility, live sperm, intact acrosome and HOST-reacted sperm in tris extender containing 20% egg yolk, 1% soy-lecithin and 1.5 % soy-lecithin was 61.20 ± 0.45, 57.77 ± 0.54 and 60.20 ± 0.45; 72.32 ± 0.47, 65.40 ± 0.56 and 67.07 ± 0.56; 68.42 ± 0.43, 61.30 ± 0.74 and 63.80 ± 0.58; and 64.35 ± 0.63, 57.35 ± 0.5 and 60.17 ± 0.46 respectively. The post thaw values of tris extender with 20% egg yolk were significantly (P<0.01) higher than that of tris extender containing 1 % and 1.5% soy-lecithin for live sperm, intact acrosome and HOST-reacted sperm, However, the difference was not significant between 20% egg yolk and 1.5% soy-lecithin for sperm motility. The post thaw values were significantly (P<0.01) higher for 1.5% than that for 1% soy-lecithin in all the parameters studied.
The overall mean post-thaw per cent sperm motility, live sperm, intact acrosome and HOST-reacted sperm for straws containing 37.5, 50 and 75 x106 sperm/ straw was 56.02 ± 0.47, 57.50 ± 0.41 and 65.57 ± 0.58; 67.42 ± 0.62, 70.55 ± 0.55 and 73.45 ± 0.57; 61.12 ± 0.69, 64.37± 0.66 and 68.25 ± 0.66; and 59.00 ± 0.62, 62.77± 0.52 and 65.57 ± 058 respectively. The post-thaw values of semen with 75 x106 sperm/ straw were significantly (P<0.01) higher than that of 50 and 37.5 x106 sperm / straw for all the sperm parameters studied. The post thaw values with 50 x106 sperm/ straw was significantly (P<0.01) higher than that with 37.5 x106 sperm /straw for sperm motility, intact acrosome and HOST-reacted sperm but not for live sperm. The fertility rate based on non-return rate was the highest at 75 x106 sperm /straw in both Beetal and Sirohi goats. It was revealed from the present study that the quality of frozen semen was superior in tris extender containing 20% egg yolk to that containing 1% or 1.5 % soy-lecithin. The post thaw quality and fertility of goat semen was significantly superior for 75 x106 sperm / straw to 50 x106 and 37.5 x106 sperm / straw.
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