DETECTION AND CHARACTERISATION OF VIRUS CAUSING SUNFLOWER MOSAIC DISEASE
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Date
2019-08-19
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UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK, BENGALURU
Abstract
Sunflower mosaic disease caused by Sunflower mosaic virus (SuMV) is one of the important viral diseases in sunflower. Sunflower plants inoculated mechanically with SuMV, exhibited mosaic symptoms followed by mottling, puckering, chlorotic rings, reduction in leaf lamina and stunted growth. Addition of 0.5 per cent sodium sulphite to phosphate buffer resulted in maximum transmission of virus. The SuMV was transmitted by Aphis gossypii with transmission efficiency of 16.00 to 24.00 per cent. The optimum pre-acquisition starvation period, acquisition feeding period and inoculation feeding periods required for successful transmission of SuMV was 60, 15 and 15 minutes respectively that resulted in 24.00 per cent transmission. In host range studies Nicotiana tabacum, Phaseolus vulgaris, Chenapodium amaranticolor and Gomphrena globosa exhibited chlorotic lesions and Cucumis sativus produced mosaic symptoms whereas, Datura stramonium and Solanum nigrum exhibited both mosaic and mottling symptoms. SuMV infected samples strongly reacted with potyvirus specific antisera in DAS ELISA. The virus was detected by RT-PCR using coat protein (CP) gene specific primers. The amplified product was ~500 bp in size. SuMV CP gene sequence analysis revealed that SuMV causing mosaic in GKVK, Bengaluru showed 98.95 per cent nucleotide identity with SuMV reported from USA and between 90-95 per cent with Sunflower chlorotic mottle virus (ScMoV) isolates reported from other countries. Out of the 11 genotypes screened under glasshouse condition, KBSH-41, KBSH-53 and AHT-5 were found to be resistant for SuMV. Among the 45 genotypes screened in field condition, only five genotypes exhibited mosaic symptoms.