In Vitro Multiplication and Preservation of Protocorm Like Bodies of Cymbidium rivulux Cooks Bridge Through Modified Culture Medium
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Date
2012
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UAS, Dharwad
Abstract
The present investigation entitled in vitro multiplication and preservation of
protocorm like bodies of Cymbidium rivulux cooks bridge through modified culture
medium, College of Agriculture, University of Agricultural Sciences, Dharwad during
the year 2010 – 2011. The investigation was carried out to find out the effect of
different treatment combinations for in vitro multiplication of PLB in terms of number
of shoots per explants, length of shoot, rooting percentage, number of roots etc. The
investigation was carried out with eight treatment and their effects on multiplication
and preservation of PLBs was measured based on the parameter studied. The results
obtained from the study exhibited that all the treatment yielded significant results for
all treatments for all the parameters and among the treatments for proliferation, T5
with BAP at 2.0 mg/l exhibit the maximum number of shoots proliferation, shoot
length, fresh weight and dry weight. In case of root proliferation T4 with NAA at 1.5
mg/l exhibited the highest rooting percentage, number of roots and length of roots. T4
with ½ M.S. + BAP 5 mg/l proved to be the most efficient treatment for in vitro
preservation (IVP) in terms of number of PLBs developed, number of plantlets per
culture so on and significant differences were found among the treatment. The
maximum PLBs development was found to be highest at 150 days of IVP, thereby
reducing trend in PLBs development was visible in subsequent days of preservation.
Further, study was carried out on the most efficient treatment culture of IVP and its
efficient treatment culture of IVP and its effect on regeneration in order to find out the
best period of IVP considering four different durations of preservation (90, 120,150
and 180 days of IVP), out of which culture obtained from 120 days of IVP where in
both treatment and different stages of observation revealed significant results. The
cost benefit ratio was assessed on the best and the least response observed from in
vitro multiplication, preservation and regeneration. In case of in vitro multiplication
T5 exhibit highest cost benefit ratio of 1:8.10 for shoot proliferation. For root
proliferation T4 exhibited the highest cost benefit ratio of 1:8.29. For in vitro
preservation T4 treatment resulted in highest cost benefit ratio of 1:3.53. Therefore, T4
treatment may be recommended for in vitro multiplication, preservation and
regeneration in future use.