Decolourization and Detoxification of Direct Blue Textile azo dye Using Bacteria

Simple item page
dc.contributor.advisorRanga, Poonam
dc.contributor.authorYogita
dc.date.accessioned2022-11-10T04:52:37Z
dc.date.available2022-11-10T04:52:37Z
dc.date.issued2021-09
dc.description.abstractIn India industrialization resulted in discharge of large amount of effluent to the environment leads to pollution. There are severely harmful impacts of dyes discharged into wastewater as well as their decomposition products are poisonous, cancerous or mutagenic to human beings & other causing health concerns (Roy DC et al., 2018). Due to their simplicity, cost-effectiveness in synthesis, firmness and range of colors of synthetic dyes are commonly used in a various industries. (Kurade et al., 2016; Ajaz et al., 2019). There are various methods to treat the industrial effluent such a physical, chemical and biological method for decolourization of azo dyes. Microorganisms play a crucial role in minerali- zation and decomposition of contaminants. Therefore, microbial decolourization of dyes has gained popularity in recent years due to their ease of rapid growth of cells and exponential rise. A total of 52 bacterial isolates were retrieved from different soil and sludge samples collected from various industrial sites. Screening of bacterial isolates was done on the basis of their ability to de- colourize Direct blue dye. In primary screening six bacterial isolates (YPR 5, YPR 13, YPR 25, YPR 34, YPR 36 and YPR 44) were selected on the basis of their decolourization potential for direct blue dye (10mgL-1). In secondary screening, decolourization efficiency of selected bacterial isolates was determined with respect to time and dye concentration. Maximum decolourization was shown by bacterial isolates YPR 36 (66.9%) and YPR 25 (62.5%) at dye concentration of 200 mgL-1 and YPR 5 (54.4%) at 100 mgL-1 after incubation period of 72 h. Optimization of cultural conditions to maximize decolourization was carried out using different parameters viz. temperature, pH and shaking/static cul- ture conditions. Under optimized conditions, maximum deolourization was observed using bacterial isolates YPR 36 (74.8%), YPR 25 (66.9%) and YPR 5 (61.6%) after 72 h. To determine the phenome- na of decolourization either by biodegradation or adsorption, both living as were as autoclaved cells of isolates were used. Selected bacterial isolates were checked for their decolourization efficiency in tex- tile effluent at different concentration (25-100%). Maximum decolourization was shown by YPR 36 (48.5%) using textile effluent concentration of 25%. Toxic effect of treated and untreated effluents was tested on Chick pea (Cicer arietinum), Green gram (Vigna radiata) and Black gram (Vigna mungo). Germination percentage, plumule and radical size were measured. Treated effluents were found to be less inhibitory to seed germination and seedling growth in comparison to untreated effluents. Based on molecular characteristics (16s rRNA gene sequencing) the promising isolates i.e. YPR 5, YPR 25 and YPR 36 were identified as Pseudochrobactrum kiredjianiae strain YPR 5, Brevundimo- nas diminuta YPR 25 and Alcaligenes faecalis YPR 36, respectivelyen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810189722
dc.keywordsDecolourization, Detoxification, Direct Blue, Textile, Azo dyeen_US
dc.language.isoEnglishen_US
dc.pages59+ xien_US
dc.publisherCCSHAU, Hisaren_US
dc.subMicrobiologyen_US
dc.themeDecolourization & Detoxification of Direct Blue Textile azo dye Using Bacteriaen_US
dc.these.typeM.Scen_US
dc.titleDecolourization and Detoxification of Direct Blue Textile azo dye Using Bacteriaen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
CCSHAU-376252-Yogita.pdf
Size:
1.93 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses