MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF POPLAR (Populus deltoides) CLONES AND DEVELOPMENT OF THEIR DESCRIPTORS
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Date
2017-11
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UHF,NAUNI
Abstract
ABSTRACT
The present study entitled “Morphological and molecular characterization of poplar
(Populus deltoides) clones and development of their descriptors” was undertaken in the
Department of Tree Improvement and Genetic Resources, Dr. Y.S. P. U. H. F., Nauni- Solan
173230 (H.P.) during year 2013-2016. The fifteen clones obtained from different research
Institutes (locations) of India. The stem cutting of 7 inch long and 1.6 cm diameter were
prepared from one year old stem cuttings and raised at a spacing of 60 cm x 60 cm in
randomized block design with three replications. The three experimental sites namely 1)
Dhaula kuan, RHTS, Dist. Sirmaur (H. P.), 2) Ladhowal farm, PAU, Ludhiana (Punjab) and
3) Nauni, Solan . Genotype x environment interaction studied during 2014 at studied sites
gave four significant parameters in leaf blade length, mid rib length, distance between petiole
junction to base of leaf and total leaf length. Therefore, stable clones obtained for leaf blade
length L-621/84, mid rib length L-621/84, distance between petiole junction to base of leaf
G-48 and total length of leaf L-621/84 and G-48. These clones should be tested in field.
Principal component analysis gave 90.05% variability for leaf traits at three different sites.
Significant Clone x Year interaction during 2013, 2014 and 2016 at Nauni was found for
ratio between mid rib to maximum width of leaf parameter. Qualitative and quantitative
descriptors for traits of poplar clones were prepared as per UPOV guidelines. Verification of
clones on the basis of SSR markers gave 64% similarity with two sub clusters.
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