Estimation of genetic diversity based on morphological and molecular markers in lentil (Lens culinaris Medikus)
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Date
2014-08
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G.B. Pant University of Agriculture & Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Lentil (Lens culinaris Medikus) is one of the versatile food legumes with diversified uses as food, fodder and fuel. The present investigation on 206 lentil genotypes was carried out in Rabi 2012-2013 to study the extent of variability, heritability and genetic advance, extent of association between yield and its components, to ascertain direct and indirect effects of component characters on seed yield, to study genetic diversity through Mahalanobis D2 Statistics based on twelve characters and assessing molecular diversity through ISSR marker.
In general, phenotypic coefficients of variation (PCV %) were greater than genotypic coefficients of variation (GCV %) and in same direction. Highest PCV % and GCV % were found for number of primary branches per plant followed by number of secondary branches per plant, economic yield per plant (gm), harvest index (HI), number of pods per plant, number of seeds per pod. Number of pods per plant, harvest index (HI), Economic yield per plant (gm) showed high values of broad sense heritability and the expected genetic advance
was observed highest for harvest index and number of primary branches.
Correlation studies showed that indicated that economic yield showed positive and highly significant at genotypic and phenotypic correlation level with number of primary branches per plant and number of pods per plant. Path coefficient analysis based on phenotypic correlation coefficient revealed that harvest index had highest positive direct effect on economic yield followed by biological yield, number of secondary branches per plant and plant height. Biological yield exhibited high positive indirect effect on economic yield followed by number of primary branches per plant followed by days to maturity, pods per cluster, number of secondary branches per plant,100 seed weight and plant height.
On the basis of Mahalanobis D2 Statistics fifteen clusters were formed. The Cluster I comprised of seventeen genotypes, Cluster II and XV comprised ten genotypes, Cluster III and XI had twelve genotypes, Cluster IV and XIII had fifteen genotypes, V and XIV consisted of eighteen genotypes, Cluster VI had twenty two genotypes, Cluster VII had nine genotypes, Cluster VIII had nineteen genotypes, Cluster IX had five genotypes, Cluster X had sixteen genotypes, Cluster XII had eight genotypes. The relative divergence of each cluster from other cluster (inter-cluster distance) indicated high degree of divergence between cluster IV and XIII, followed by cluster IV and XIV. Number of pods per plant contributed maximum towards genetic divergence followed by number of secondary branches per plant, biological yield, number of primary branches per plant, number of seeds per pod, 100-seed weight, harvest index and economic yield.
In the present study 14 ISSR primers were taken, out of them five primers gave amplification. These primers gave a total of 28 loci, all primers are polymorphic. A dendrogram generated by cluster analysis derived from ISSR markers divided 24 lentil genotypes into two group A and B. Major group A have 22 genotypes and major group B have 2 genotypes. EC78511 and EC267528-B were most similar and EC223191 and EC78511 were most distant. This Indicates ISSR as a useful tool in determining the genetic diversity among genotypes in lentil because it is not influenced by environmental conditions.
Description
Thesis-PhD
Keywords
genetic diversity, molecular markers, lentils, lens culinaris, plant morphology, food legumes, yield components, correlation, economic yield, heritability