A study on milk proteome and biochemical analysis of milk and blood of mastitic and healthy Sahiwal cows
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Date
2016-12-26
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U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go‐Anusandhan Sansthan Mathura-281001
Abstract
The study was performed at Department of Veterinary Biochemistry, College of Veterinary Sciences and Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go- Anusandhan Sansthan, (DUVASU), Mathura, Uttar Pradesh, India. The study was carried out on lactating Sahiwal cows maintained at ILFC of the College of Veterinary Science, Mathura. Milk samples used in this study were of control/apparently healthy group, subclinical mastitis and clinical mastitis group.
The animals were classified into three stages of lactation namely, Early lactating group (up to 100 days), Mid lactating (from 101 to 200 days) and late lactating (from 201 to 280 days). Now these groups were further divided in to three subgroups, namely control/healthy group subclinical mastitis and clinical mastitis group on the basis of screening by using somatic cell count (SCC) , California mastitic test (CMT) and Electrical conductivity test (ECT) on the milk samples.
Biochemical analysis of milk samples revealed significant increase in Sodium, copper, MDA, Lactose, LDH, AST and ALP and decrease in Magnesium, calcium , phosphorus, potassium, iron, zinc, Total protein and Albumin compared to healthy animals.
Milk of healthy animal showed significant decrease in Sodium and LDH whereas Subclinical mastitic animals revealed significant fall in copper and LDH and rise in copper levels in Mid and Late lactation compared to early lactation.
Biochemical study of serum of healthy, SCM and CM Cows showed significant increase in Sodium, potassium, Total protein, Globulin LDH and AST while significant decrease in calcium, copper, albumin, A/G ratio, FFA, MDA and Cholesterol.
The stage of lactation found to affect biochemical profile in both serum and milk and revealed significant increase in Calcium, phosphorus, copper, glucose, cholesterol, LDH and AST and decrease in Total protein in Mid and Late lactation compared to early lactation in healthy animals. Similarly cows having SCM showed significant increase in calcium, phosphorus, sodium, glucose and cholesterol and decrease in Total protein and MDA in Mid and Late lactation.
Bacterial isolation and multiplex PCR performed on milk samples of SCM/CM revealed infection of Staphylococcus, Pseudomonas and E. coli in the studied cows.
SDS PAGE analysis of pooled milk samples healthy milk revealed 23 protein bands where as both subclinical and clinical mastitic milk 27 protein bands. The 4 protein bands which were not found in the healthy milk revealed 14, 56, 160 and 168 kDa molecular weight proteins.
2-D gel electrophoresis was carried out by using isolated whey proteins of healthy SCM and CM sahiwal cows. IEF and I-D gel analysis revealed expression of large set of proteins but on gel analysis revealed 3 differentially expressed protein spots in CM group of proteins. Those spots were identified, digested and sent for MALDI analysis. MALDI analysis revealed following important proteins.
a) DNA-dependent protein kinase catalytic subunit, protein.
b) Rho-specific guanine nucleotide exchange factor (RhoGEF)
c) acyl-CoA-binding domain-containing protein 6 isoform X4 [Bubalus bubalis]
d) Ankyrin repeat domain-containing protein [Homo sapiens]
e) Chordin-like protein 1
f) kielin/chordin-like (KCP) protein
Description
A study on milk proteome and biochemical analysis of milk and blood of mastitic and healthy Sahiwal cows
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