Development and Exploitation of Heterotic pools of Hirsutum and Barbadense for Developing Potential Inter Specific Hybrods, Molecular Marker and Genetic Transformation Study in Cotton.
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Date
2013
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UAS Dharwad
Abstract
Reciprocal selection for improving combining ability was modified to suit cotton
and tested. Based on earlier studies DB 533 and DB 534 the selected barbadense elite
combiners were crossed and advanced to F4 generation for creating recombinational
variability for combining ability against the hirsutum testers (DH 98-27, ZCH 8, 178-24 and
DH 18-31). In line x tester study, DB 533 and DB 534 were compared with barbadense lines
in developing productive inter specific hybrids. Both of them recorded positive gca effects
for seed cotton yield confirming potentiality to form heterotic groups of hirsutum vs
barbadense cottons. Of the 23 hirsutum testers, four testers mentioned above exhibited
positive gca values for yield. The potentiality of the heterotic box was also confirmed by
comparing these eight bench mark crosses with 49 inter specific crosses and checks. Among
the 53 F5 barbadense lines, DB 533 x DB 534 F5 IPS 18 showed exceptional superiority for
productivity and fiber quality traits.
Among three methods of determining pooled score, weighted percent gca
approach was efficient in identification of potential combiners. Based on mean and coefficient
of variability for productivity DH 98-27 was found to be efficient tester. Sub
grouping the F4 lines against pairs of hirsutum testers was done. Among the population used
four F4 lines revealed transgressive positive segregation for combining ability against all the
four testers and the lines DB 533 x DB 534 F4 IPS 49 and DB 534 x DB 533 F4 IPS 22 were
selected for developing sub populations against hirsutum testers. With the help of 40 SSR
markers, molecular diversity was assessed and positive correlation was found between
genetic distance (GD) and seed cotton yield of F1 and heterosis over checks. In vivo
transformation was attempted in hirsutum variety.