Validation of QTLs for Yellow Mosaic Virus Tolerance in Mungbean (Vigna radiata (L.) Wilczek)
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Date
2018
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Acharya N.G. Ranga Agricultural University
Abstract
Green gram (Vigna radiata (L.) Wilczek), is one of the important
pulse crops mainly grown in developing countries. However, the yield level
of the crop is very low due to many biotic and abiotic factors. Among biotic
factors, yellow mosaic virus (YMV), which is transmitted by white fly
(Bemisia tabaci) causes significant yield losses ranging from 10-100%. With
this background, the present investigation was initiated to validate the
reported markers linked to the YMV resistance in a F2 segregating
population. The genotypes, TM-96-2 (Trombay Mungbean), susceptible to
Yellow Mosaic Virus (YMV) and EC-396117 (Exotic Collection) tolerant to
YMV were chosen as parents for development of F2 population.
The parental DNA was screened with 206 markers which include
microsatellites (203), RAPD (2) and SCAR (1) to detect the polymorphic
markers. Out of these markers, 110 were amplified and 73 markers were not
amplified. Of these 110 amplified markers, only 16 primers showed
polymorphism (14.5%) between the parents, and the rest of the markers
were found to be monomorphic. True F1 plants were selected after
confirming their heterozygosity at molecular level using polymorphic
microsatellite marker, CEDG305. These F1s were selfed to produce F2
population during Rabi, 2017-2018.
As many as 150 F2 individuals along with the parents were evaluated
for YMV tolerance at RARS, Tirupati during Summer, 2018 under natural
field conditions. The DNA has been extracted from the parents and F2
population. All the 150 F2 plants were screened with the 16 polymorphic
markers. Out of these markers, only 5 showed clear scorable bands in F2
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population. These 5 markers were CEDG245, CEDG149, CEDG305,
DMBSSR125 and CEDG228. Single marker analysis of these five markers
in the F2 population using MapDisto software v.2.0 revealed a significant
association of CEDG228 marker with YMV tolerance. NCBI-BLAST
analysis of primer sequence of CEDG228 hit an expressed gene,
LOC106773961 of the greengram genome.
In conclusion, the marker, CEDG228, which has shown association
with the YMV tolerance in the present investigation, has the potential to use
in marker-assisted breeding of the development of YMV tolerant green gram
varieties. However, further validation of this marker in a diverse set of
resistant and susceptible cultivars is warranted before being used in large
scale marker-assisted selection programmes.
Description
D5723
Keywords
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