Development of Microbial Consortium of P- solubilizing Plant Growth Promoting Rhizobacteria and Its Effect on Onion
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Date
2016
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Mahatha Phule Krishi Vidyapeeth,Rahuri
Abstract
solubilizing plant growth promoting rhizobacteria (PGPR) from the rhizosphere soils of onion crop and to develop a protocol for efficient phosphate solubilizing PGPR consortium in the form of liquid bio-formulation evaluate their effect on onion, and analyze the molecular diversity within the group of PGPR isolates.
In the present study, a total of 32 isolates which were able to solubilize rock phosphate, through the production and excretion of organic acids were obtained from the rhizosphere soils of selected places of Western Maharashtra viz., Pune, Satara, Solapur, Ahmednagar, Nasik districts etc. The commercially used phosphoroussolubilizing bacterial strain was used for comparison from Vasantdada Sugarcane Institute (VSI) Pune.
A total no. of 32 phosphate solubilizing PGPR isolates were isolated. Out of 32 P- solubilizing PGPR isolates, 26 isolates showed zone of phosphate solubilization at 48 hours after incubation (HAI) and it ranged from 9.00 mm to 24.94 mm. Among all the isolates P-PGPR 21, P-PGPR 24 and P-PGPR 43 recorded maximum zone of solubilization (24.94 mm, 23.20 mm and 21.61 mm, respectively) than the other isolates and also commercially used phosphate solubilizing bacterium (CPSB) 20.75 mm. Out of 26 isolates, 20 isolates showed more than 13 mm zone of phosphate solubilization from TCP.
On the basis of screening of phosphate solubilizers, out of 26 isolates, 20 isolates were found more efficient in phosphate solubilization zone and phosphate solubilizing activity in Pikovaskaya’s broth. These 20 bacterial isolates were further screened for their siderophore and IAA production ability and also for phosphate solubilizing ability quantified under pot trial study. Only 13 bacterial isolates showed best performance in pot study and were positive in siderophore and IAA production test.
The Phosphate solubilizing PGPR isolates were designated as P- PGPR. Based on the morphological, cultural, physiological and biochemical tests, the selected bacteria were identified at genus level as Pseudomonas sp., Bacillus sp. and Azotobacter sp. Out of the 13 isolates, 6 were to be found Pseudomonas sp., 5 were Bacillus sp. and 2 Azotobacter sp.