Studies on genetic variability in Snapmelon (Cucumis melo L. Var momordica ) through physic-chemical traits and seed protein profiling
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Date
2013-04-04
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College of Horticulture and Forestry, Central Agricultural University, Pasighat
Abstract
Analysis of variance revealed significant differences among the genotypes for all the attributes. The genotypes CHFSM-5, CHFSM-10 CHFSM-11 and CHFSM-15 were found to be superior for the most of the yield components and fruit quality traits. High PCVB and GCV, heritability, genetic gain were observed for Days to 50% germination, fruit length, fruit weight, flesh thickness, number of fruit per plant, number of seed per fruit, TSS, ascorbic acid, titrable acidity and fruit yield per plant.
Correlation studies indicated that fruit yield pr plant was positively and significantly correlated with vine length, nmbser of primary branches per vine, fruit length, fruit diameter, fruit weight, flesh thickness numnbse of fruits per plant, and number of seed per fruit however negative and significant association was established with TSS and titrable acidity.
Maximum positive direct effect on fruit yield per plant was implosed ty TSS, number of seed per fruit, flesh thickness, fruit weight and fruit length which were observed as the most important traits affecting fruit yield per plant, However, high negative direct effect was observed for days to first staminate flower anthesis, number of fruits per plants, and days to first pistilate flower anthesis.
Divergence study revealed that ascorbic acid content contributed maximum percent to diversity followed by titrabble acidity, fruit yield per plant, number of seed per fruit, and TSS. Maximum inter cluster distance was observed between cluster 4 and 5 and indicated that the genotypes within these were highly divergent.
SDS-PAGE analysis showed a total of 54 bands identified by coomassie brilliant blue staining. The genotypes exhibited considerable variation in protein band number ranging from 19-31. Among the genotypes CHFSM-1 and CHFSM-8 showed maximum number (31) of protein bands while the minimum numbers (19) of bands were present in CHFSM-2, CHFSM-11, CHFSM-12 and CHFSM-17.
Description
The present investigation was conducted during summer 2012 at vegetable Research Farm, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh in 20 diverse genotypes of snapmelon. The experiment was laid out in randomized complete block design with three replications. Genetic variability was studied for attributes viz, days to 50% germination, vine length, number of primary branches, days to first staminate flower anthesis, days tofirst pistilate flower anthesis, days to first harvest, fruit length, fruit diameter, fruit weight, flesh thickness, number of fruits per plant, number of seed per fruit, TSS, ascorbic acid, titrable acidity and fruit yield per plant. Data were analyzed statistically for phenotypic and genotypic variance, coefficient of variation, heritability, genetic advance, genetic gain, correlation coefficient, path coefficient, genetic divergence and seed protein banding pattern
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