Synthesis, Characterization and Evaluation of Coppera and Sulphur Nanoparticles Against Major Plant Pathogens
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Date
2018-03
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University of Agricultural Sciences, Dharwad
Abstract
Green nanotechnology is the current requirement in plant pathology and considering the magnitude of this novel technology, investigations were undertaken on
green synthesis of copper nanoparticles (CuNPs) and sulphur nanoparticles (SNPs), characterization and evaluation against the major plant pathogens to mitigate the disease problem effectively both in vitro and in vivo condition at College of Agriculture, University of Agricultural Sciences, Dharwad. Green syntheses of NPs were attempted from seven plants by six methods in copper and two methods in sulphur. Particle Size Analyzer confirmed the average diameter of nanoparticles was < 100 nm [CuNPs (32.5 nm) and SNPs (56.00 nm)]. Circular to irregular in shape of NPs was confirmed by AFM and SEM.
Recovery of CuNPs and SNPs was highest in pome granate pericarp mediated synthesis. Major five fungal and three bacterial plant pathogens were isolated by
standard procedure and characterized by molecular techniques. CuNPs inhibited the cent per cent spore germination of Exserohilum turcicum at 500 ppm. SNPs showed cent per cent inhibition of spore germination of Golovinomyces cichoracearum at 10 ppm. Mango leaf based CuNPs inhibited the growth of Xanthomonas axonopodis pv. punicae (17.33 mm) and Ralstonia solanacearum (12.00 mm) at 2,000 ppm. In chickpea, SNPs at 1,000 ppm recorded highest seed quality parameters by paper towel method and was on par with carboxin (37.5 %) + thiram (37.5 %). Under glasshouse condition, SNPs at 500 and 1,000 ppm managed the greengram and cowpea powdery mildew diseases respectively. Citrus canker and bacterial wilt of tomato diseases were significantly managed by CuNPs over untreated control and remained on par with standard antibiotic check.
Green synthesized CuNPs and SNPs were found effective in reducing the diseases caused by major fungal pathogens compared to bacteria at lower concentrations. No phytotoxic symptoms were observed on tomato seedlings at 2000 ppm.