UV PROTECTION AND SHELF LIFE STUDIES OF HaNPV
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Date
2018-06-14
Authors
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Publisher
Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
The present investigation was undertaken to study the “UV protection and shelf life studies of HaNPV” under laboratory conditions during the year 2017-18 in Bio-control laboratory, Department of Agricultural Entomology, PGI, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Efforts were made to find effect of UV light on Virulence of HaNPV and to find out effective UV protectant for HaNPV.
The viability of HaNPV was recorded by taking the POB count under the microscope after every 15 min exposure to UV source of 15 W 54 volts UV light in a UV chamber under the laboratory conditions. The results revealed reduction in POB activity up to 50.71, 59.86, 64.79, 71.84% after 15, 30, 45 and 60 min of UV exposure, respectively. The Mortality data on Helicoverpa larvae recorded at 7th day after treatment revealed 69.44, 66.66, 50.00 and 58.33% larval mortality by HaNPV after 15,30,45 and 60 exposures to UV rays, indicating 30.6, 33.34, 50.00 and 41.67% reduction in mortality as compared to initial HaNPV sample. Out of the nine plant extracts screened for their UV protection property, Ashwagandha, Mango, Eucalyptus and Aonla leaf extract were found effective recording less reduction in POBs and increased Helicoverpa mortality.
The shelf life of HaNPV kept under laboratory condition was assessed after every month for a period of 6 months and the data revealed reduction in POBs as the initial count of 1.42 × 109 POBs/ml was reduced down to 1.02 × 109 POBs/ml after six months period indicating 28.17% reduction in sole HaNPV sample.
Description
The present investigation was conducted to evaluate the efficacy of NPV against H. armigera. UV light adversely affect the performance of HaNPV recording reduction in POB count up to 70 % and mortality up to 58% and therefore there is a necessity to add UV protectants while using under field condition. Polyflavonoids (Mango leaf extract, Aonla leaf extract, Eucalyptus and Ashwagandha leaf extracts) provide best protection for HaNPV from UV- radiation (280 to 320) and maintain its virulence for longer period and can be used under field conditions. The shelf life particularly the POB count of a stored HaNPV suspension was found to reduce upto 28% when stored for 6 months under room temperature.
Keywords
Plant Protection, Control Methods, Biocontrol, Biological control agents, Crops, Field crops, Cicer arietinum, Cajanus cajan, Helicoverpa armigera, Nuclear Polyhedrosis Virus, HaNPV, Ultravoilet radiation, UV Protectants, Nucleopolyhedrovirus, Photoprotection, Shelf life, Species specificity, Persistance, Plant extracts, Antioxident property,
Citation
KHARVIND, KIRAN SHIVNANDAN. (2018). UV protection and shelf life studies of HaNPV. Department of Agricultural Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2018. Print. xii, 60p. (Unpublished).