Antioxidative defense mechanism in wild and cultivated chickpea cultivars against Helicoverpa armigera infestation
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Date
2019
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Punjab Agricultural University, Ludhiana
Abstract
The present study was undertaken to study the biochemical response in leaves, pod wall and seeds of three wild (GLW 22, GLW 58 and GLW 187) and five desi (GL 12021, GL 29095, GL 29078, H11 22 and CSJ 515) chickpea genotypes by comparing them with two checks (resistant check ICCL 86111 and susceptible check ICC 3137) against Helicoverpa armigera infestation. Superoxide dismutase activity significantly increased in leaves of resistant check ICCL 86111 after infestation. Catalase and peroxidase activities increased in leaves of GLW 58, GL 12021, GL 29095 and GL 29078 and ascorbate peroxidase-glutathione reductase activities increased in leaves of GLW 58 after infestation as compared to resistant check. Higher activities of superoxide dismutase, catalase and peroxidase was observed in podwall of GLW 187 and GL 29078. Higher proline content in leaves of GL 29078; in podwall of GLW 22, GL 12021 and H11 22 and in seeds of GLW 187, H11 22 and GL 29078 after infestation might be due to the enhanced activities of proline biosynthetic enzymes in them. Higher activities of polyphenol oxidase in leaves of GLW 22 and GL 29095 as well as enhanced activities of phenylalanine ammonia lyase was observed in leaves of all the genotypes as compared to resistant check ICCL 86111. MDA content decreased in leaves, podwall and seeds of GLW 187 and GL 12021 after infestation. Higher flavanol and total phenol content was observed in leaves of GLW 22, GLW 187, H11 22 and GL 29078 after infestation. Lower percent pod damage (9.58-12.44%) in GL 12021, GL 29095, H11 22, GLW 22, GL 29078, GLW 187 as compared to pod damage in resistant check (16.18%) and susceptible check (21.50%) might be attributed to differential induced defense mechanism in them.
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