PROGENY ANALYSIS IN WILD POMEGRANATE (Punica granatum L.)

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Date
2018-07
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UHF,NAUNI
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ABSTRACT The study entitled “Progeny analysis in Wild Pomegranate (Punica granatum L.)” was conducted in the Department of Tree Improvement and Genetic Resources during the year 2015-17 with the aim to evaluate the selected parent trees, fruits and nursery performance of the half sibs and full progeny. Selection of the trees based on fruit characteristics and pedigree analysis of hybrids using RAPD from the promising progenies. Three locations were randomly selected viz. Tatool (L1) district Solan, Narag (L2) and Daron (L3) district Sirmour (H.P). The fruit samples from Tatool location proclaimed assuring fruit length (56.36 mm) and width (53.30 mm) in tree number 2 with ovate shape which also has a promising average fruit weight of 47.43 g greater than the mean of all the locations with a desirable TSS value of 17.13. Maximum value in fruit traits were obtained from open pollination as compared to controlled cross combination for all the characters except fruit weight and fresh seed weight with aril. The maximum fresh seed weight with aril was recorded from genotype L3T2 having ovate fruits. The nursery stage growth of the half sib seedlings from L3T3 and L3T5 have shown promising mean height of 62.05 cm and highest mean collar diameter of 5.32 mm respectively. So, the half sibs from Daron location performed best for seedling height as compared to the half sibs from other location. The fruit borer incidence of 9.67 % was recorded from Daron location tree number 1, bearing oval fruits followed by two other genotype with oval shape also resulted in lesser incidence of the borer. The highest titrable acidity was recorded from genotype L3T3 with elliptical shape which is beneficial for Anardana/condiments processing. The tree genotypes bearing ovate and elliptical shaped fruits can be preferred for better fruit size selection. High heritability was observed for percent germination and seedling height at six month age. Reputed fresh weight of seed without aril and germination percent was observed in L2T2 X L3T3 crosses indicating favourable growth of the seedlings. The cross combination L1T1 X L2T1 has proved to be better with respect to fruit quality biochemical estimates. The fruit from the crosses viz. L2T4 X L2T1 and L2T2 X L2T1 have shown lesser incidences of the borer attack. High genotypic coefficient of variability, heritability, genetic advance and genetic gain were observed for fresh weight of fruit, fresh rind weight, rind thickness and number of leaves per seedlings. The hybrids so obtained were tested for their pedigree using 21 RAPD markers of which 3 primers revealed the clue for hybridity. RAPD banding pattern obtained after the amplification of genomic DNA of parent trees and their seven hybrids expressed the similar patterns.
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