Characterization of “Land mark Protein” from Rhizobium isolate of Pigeon pea collected from the acidic soil regimes of the State Of Jharkhand
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Date
2018
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Birsa Agricultural University, Ranchi, Jharkhand-6
Abstract
Agriculture sector is the backbone of Indian economy however; agricultural productivity is very
low in some countries. The low productivity is due to decreasing soil fertility many factors
responsible for decreasing soil fertility such as soil acidity, soil erosion, and continuous
cropping. Soil acidity occurs when there is a buildup of acid forming element. Acidic soil in
Jharkhand occupies approximately 90 percent area of the land. Most leguminous plant requires
neutral or slightly acidic soil for growth, especially when depending on symbiotic nitrogen
fixation.
Pigeon pea is a legume, improves soil fertility through biological nitrogen fixation. It is reported
to contribute approximately 40 kg N ha-1 soil acidity is one of the serious problem which affect
growth of rhizobia in the soil of Jharkhand Rhizobium is a gram negative bacteria, soil living
bacteria. Rhizobium provides organic nitrogenous compound to the plant and plant provide
organic compound made by photosynthesis, this relation is known as symbiosis.
Proteomics is a large scale study of proteins. Two-dimensional gel electrophoresis is
commonly used to analyse proteins. In 2-D gel electrophoresis proteins are separated in two
dimensions on the basis of two independent properties i.e. Isoelectric point [pI] (1st Dimension
(Isoelectric focusing (IEF)) and Molecular weight [MW] (2nd Dimension (SDS-PAGE)) In this
study we perform 2-DE electrophoresis to establish the spots reproducibility of the protein In our
present study we find that protein changes between isolates from normal pH and acidic pH
intervals. Lot of proteins change in form of prominent spots which were altered across the
various isolates collected from various pH regimes. Such changes reflect the adaptative changes
which are under play at protein level for helping the bacteria survive under harsh acidic pH
regimes. On the other hand, it is also a possibility that such changes reflect many other protein
changes which arise out of processes, such as post-translational modification and the presence of
isoforms.
Reproducible land mark protein spots were observed in the gels by 2D electrophoresis by
utilizing 2-DE approach subsequently protein which might be implicated to play crucial roles in
imparting acid soil-tolerance to Rhizobium isolates of pigeon pea were identified from the
isolates collected acid soils of the state of Jharkhand, various spots of Rhizobium isolates were
analyzed Protein play a major role to cope with abiotic stress.
Description
Characterization of “Land mark Protein” from Rhizobium isolate of Pigeon pea collected from the acidic soil regimes of the State Of Jharkhand
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