Evaluation of Blood Constituents as Diagnostic Markers for Ethmoid Carcinoma in Cattle
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Date
1993-08-03
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES Mannuthy - Thrissur
Abstract
An investigation was carried out to evaluate the
usefulness of blood constituents as tumour markers for
carcinoma of ethmoid mucosa in cattle. During the period of
two years, twenty nine tumour bearing cattle in various
stages of ethmoid carcinoma procured from different parts of
Kerala and twelve clinically healthy animals maintained at
the University Livestock Farm, Mannuthy {controls) were bled
and serum samples were collected.
Serum calcium, serum phosphorus and serum magnesium
levels of tumour animals were compared with that of the
control animals. Tumour animals had a mean serum calcium
level of 11.78+0.23 mg per cent, mean serum phosphorus level
of 6.11+0.10 mg per cent and mean serum magnesium level of
2.35+0.05 mg per cent. In control animals the mean serum
calcium, phosphorus and magnesium levels were 10.23+0.10 mg
per cent, 6.66+0.09 mg per cent and 2.27+0.07 mg per cent
respectively. There was increase in the serum calcium level
and reduction in serum phosphorus level in tumour bearing
animals was statistically significant. The serum magnesium
level did not reveal any statistical significance.
The mean serum total sialic acid and mean serum lipid
bound sialic acid levels in tumour bearing animals was
95.21+0.78 ng/dl and 30.83+0.36 ng/dl respectively and in
control animals it was 60.67+0.87 ng/dl and 11.67+0.43 ng/dl
respectively. Both serum total sialic acid and serum lipid
bound sialic acid level in tumour bearing animals was high
when compared with that of control animals and the increase
was statistically significant.
Serum from twenty~nine tumour bearing animals and serum
control animals were tested against the prepared tumour
antigen using agar gel precipitation test for detecting
tumour antibodies. All the twenty nine serum samples from
tumour animals showed two distinct precipitin bands, one
band close to the serum well and another sharp band closer
towards the antigen well. Majority of the control animal
serum samples also showed both the precipitin bands although
some of the control samples did not show the second sharp
band nearer to the antigen well.
Passive haemagglutination test was conducted using
tumour antigen sensitized gluteraldehyde stabilized sheep
red blood cells, tumour animal serum control animal serum
and proper controls. The highest titre value obtained for
serum of tumour animals was 1:256 and the lowest was 1:8.
In control animals it was 1:128 and 1:32 respectively for
highest and lowest titre values. Since in the control
animals the titre value showed only the difference of one
well the comparison of the control and tumour animals titre
values indicated that a base line cut off value cannot be
obtained to differentiate the tumour infected and control
animals. None of the tumour samples processed and
inoculated into embryonated eggs revealed the presence of
any haemagglutinating agents.
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